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. 1967 Jan;42(1):67–75. doi: 10.1104/pp.42.1.67

Properties of Higher Plant Mitochondria. I. Isolation and Some Characteristics of Tightly-coupled Mitochondria from Dark-grown Mung Bean Hypocotyls 1

Hiroshi Ikuma 1,2, Walter D Bonner Jr 1
PMCID: PMC1086490  PMID: 16656487

Abstract

The mitochondria isolated from dark-grown mung bean hypocotyls oxidize succinate, l-malate, and externally added reduced nicotine adenine dinucleotide (NADH) with good respiratory control. While the pattern of respiration resembles that of animal mitochondria, there are 4 basic differences between the respiratory properties of mung bean and animal mitochondria: A) the ability to oxidize NADH, B) the pattern of succinate and malate oxidation, C) the rate of oxygen uptake, and D) the adenosine-5′-diphosphate to oxygen ratios.

The apparent `Km' for malate of mung bean mitochondria is about one order higher than that expected from malic dehydrogenase in animal mitochondria, whereas the affinity for phosphate is about 5 times higher with plant mitochondria than rat-liver mitochondria. While the half-maximal stimulation of respiration by adenosine-5′-diphosphate is practically identical to that of animal mitochondria, higher concentrations of adenosine-5′-diphosphate cause some decrease in its stimulating action.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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