Abstract
The effects of various inhibitors of nucleic acid and protein synthesis on protochlorophyllide synthesis in dark-grown Phaseolus vulgaris var. Red Kidney have been studied. Actinomycin D, chloramphenicol, and puromycin inhibit the regeneration of protochlorophyllide holochrome (detected as a 650 mμ absorption peak) in vivo in darkness after photoconversion of endogenous protochlorophyllide a to chlorophyllide a; this inhibition does not occur in similarly treated leaves supplied with δ-aminolevulinic acid.
These data suggest that the regeneration of protochlorophyllide results from the synthesis of RNA and enzymes required for the production of δ-aminolevulinate.
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Selected References
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