Abstract
Some microscope observations of the protein bodies of the cotyledon cells of the soybean (Glycine max) are described, together with changes in their appearance which occur on germination. Density gradient centrifugation permits the isolation of protein bodies from soymeal. They contain about 70% of the protein of the bean. Only 1 protein could be detected in them: glycinin, the major soybean protein.
The protein bodies were fractionated to light and heavy fractions. The former contained 97.5% protein, the latter 78.5%. RNA, phytic acid and lipids were also present. The 2 fractions probably differ only in the extent of contamination by other cell fragments.
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- ALTSCHUL A. M., SNOWDEN J. E., Jr, MANCHON D. D., Jr, DECHARY J. M. Intracellular distribution of seed proteins. Arch Biochem Biophys. 1961 Nov;95:402–404. doi: 10.1016/0003-9861(61)90166-7. [DOI] [PubMed] [Google Scholar]
- Bragg P. D., Hough L. An investigation of the egg-white mucoproteins, ovomucoid and ovalbumin. Biochem J. 1961 Jan;78(1):11–23. doi: 10.1042/bj0780011. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horner H. T., Jr, Arnott H. J. A histochemical and ultrastructural study of yucca seed proteins. Am J Bot. 1965 Nov-Dec;52(10):1027–1038. [PubMed] [Google Scholar]
- Morton R. K., Palk B. A., Raison J. K. Intracellular components associated with protein synthesis in developing wheat endosperm. Biochem J. 1964 Jun;91(3):522–528. doi: 10.1042/bj0910522. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Varner J. E., Schidlovsky G. Intracellular Distribution of Proteins in Pea Cotyledons. Plant Physiol. 1963 Mar;38(2):139–144. doi: 10.1104/pp.38.2.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WOLF W. J., BRIGGS D. R. Purification and characterization of the 11S component of soybean proteins. Arch Biochem Biophys. 1959 Nov;85:186–199. doi: 10.1016/0003-9861(59)90462-x. [DOI] [PubMed] [Google Scholar]