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. 1967 Jul;42(7):907–910. doi: 10.1104/pp.42.7.907

Effect of 2′-Deoxyadenosine on Thymidine Utilization by Tradescantia Pollen Grains 1

S T Takats 1
PMCID: PMC1086648  PMID: 16656595

Abstract

The uptake of 3H-thymidine into pollen grains of Tradescantia paludosa was studied in the presence of 2′-deoxyadenosine. 1) Millimolar deoxyadenosine caused an immediate inhibition of incorporation of 3H-thymidine into DNA extracted with hot trichloroacetic acid. 2) The radioactivity in acid-soluble derivatives of 3H-thymidine was examined by paper chromatography and, following incubation of pollen grains in the presence of millimolar deoxyadenosine, was found to be increased several-fold in 3H-deoxythymidine triphosphate. 3) The time-course of inhibition showed that the acid-soluble derivatives of 3H-thymidine accumulated initially at a rate unaffected by deoxyadenosine, despite the nearly complete inhibition of incorporation of 3H-thymidine into DNA. This is discussed in relation to possible mechanisms of inhibition by deoxyadenosine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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