Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1967 Sep;42(9):1229–1238. doi: 10.1104/pp.42.9.1229

Structure and Function of Tomato Leaf Chloroplasts During Ammonium Toxicity 1

George S Puritch 1,2, Allen V Barker 1
PMCID: PMC1086708  PMID: 16656644

Abstract

Ammonium toxicity resulted in morphological modifications of tomato leaf chloroplasts. The chloroplasts, which are normally flattened around the protoplast periphery, became ellipsoidally rounded and dispersed through the protoplasm. The first apparent effect of plastid degradation was development of many vesicles from the fretwork. Later the grana lamellae swelled, and some disappeared. Eventually, distinct grana could not be detected.

Ammonium accumulation, chlorophyll loss, and photosynthetic decrease occurred simultaneously. Initial changes in these processes preceded the detection of modifications of fine structure; however, each continued with further breakdown of the chloroplasts.

Full text

PDF
1229

Images in this article

1231Image
on p.1231
1233Image
on p.1233

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AVRON M. Photophosphorylation by swiss-chard chloroplasts. Biochim Biophys Acta. 1960 May 20;40:257–272. doi: 10.1016/0006-3002(60)91350-0. [DOI] [PubMed] [Google Scholar]
  2. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barker A. V., Volk R. J., Jackson W. A. Root environment acidity as a regulatory factor in ammonium assimilation by the bean plant. Plant Physiol. 1966 Sep;41(7):1193–1199. doi: 10.1104/pp.41.7.1193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CARELL E. F., KAHN J. S. SYNTHESIS OF PORPHYRINS BY ISOLATED CHLOROPLASTS OF EUGLENA. Arch Biochem Biophys. 1964 Oct;108:1–6. doi: 10.1016/0003-9861(64)90347-9. [DOI] [PubMed] [Google Scholar]
  5. Izawa S., Good N. E. Effect of Salts and Electron Transport on the Conformation of Isolated Chloroplasts. II. Electron Microscopy. Plant Physiol. 1966 Mar;41(3):544–552. doi: 10.1104/pp.41.3.544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kirk J. T., Allen R. L. Dependence of chloroplast pigment synthesis on protein synthesis: effect of actidione. Biochem Biophys Res Commun. 1965 Dec 21;21(6):523–530. doi: 10.1016/0006-291x(65)90516-4. [DOI] [PubMed] [Google Scholar]
  7. Krogmann D. W., Jagendorf A. T., Avron M. Uncouplers of Spinach Chloroplast Photosynthetic Phosphorylation. Plant Physiol. 1959 May;34(3):272–277. doi: 10.1104/pp.34.3.272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LYTTLETON J. W., TS'O P. O. The localization of fraction I protein of green leaves in the chloroplasts. Arch Biochem Biophys. 1958 Jan;73(1):120–126. doi: 10.1016/0003-9861(58)90246-7. [DOI] [PubMed] [Google Scholar]
  10. MERCER F. V., NITTIM M., POSSINGHAM J. V. The effect of manganese deficiency on the structure of spinach chloroplasts. J Cell Biol. 1962 Nov;15:379–381. doi: 10.1083/jcb.15.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PACKER L., MARCHANT R. H., MUKOHATA Y. Observations on the control of chloroplast structure by adenosine triphosphate. Biochem Biophys Res Commun. 1963 Jun 20;11:429–434. doi: 10.1016/0006-291x(63)90087-1. [DOI] [PubMed] [Google Scholar]
  12. PACKER L. STRUCTURAL CHANGES CORRELATED WITH PHOTOCHEMICAL PHOSPHORYLATION IN CHLOROPLAST MEMBRANES. Biochim Biophys Acta. 1963 Jul 23;75:12–22. doi: 10.1016/0006-3002(63)90574-2. [DOI] [PubMed] [Google Scholar]
  13. PARK R. B., PON N. G. Correlation of structure with function in Spinacea oleracea chloroplasts. J Mol Biol. 1961 Feb;3:1–10. doi: 10.1016/s0022-2836(61)80002-8. [DOI] [PubMed] [Google Scholar]
  14. Racusen D., Foote M. Protein Turnover Rate in Bean Leaf Disks. Plant Physiol. 1962 Sep;37(5):640–642. doi: 10.1104/pp.37.5.640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. TREBST A. V., LOSADA M., ARNON D. I. Photosynthesis by isolated chloroplasts. XII. Inhibitors of carbon dioxide assimilation in a reconstituted chloroplast system. J Biol Chem. 1960 Mar;235:840–844. [PubMed] [Google Scholar]
  16. VERNON L. P., ZAUGG W. S. Photoreductions by fresh and aged chloropasts: requirement for ascorbate and 2, 6-dichlorophenolindophenol with aged chloroplasts. J Biol Chem. 1960 Sep;235:2728–2733. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES