Abstract
Electron transport activity and absorbance changes associated with P700 were investigated in a mutant strain of Chlamydomonas reinhardi with impaired photosynthesis. This mutant strain, ac-8oa, cannot reduce NADP with electrons from either water or dye and ascorbate, but it has considerable Hill activity. The mutant strain shows none of the absorbance changes characteristic of P700. Although unable to carry out cyclic photosynthetic phosphorylation, ac-8oa is able to synthesize ATP when ferricyanide is provided as an electron acceptor.
These observations lead to the conclusion that a site for the coupling of photosynthetic phosphorylation with electron transport must exist between the 2 photochemical systems.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AVRON M. Photophosphorylation by swiss-chard chloroplasts. Biochim Biophys Acta. 1960 May 20;40:257–272. doi: 10.1016/0006-3002(60)91350-0. [DOI] [PubMed] [Google Scholar]
- AVRON M., SHAVIT N. A SENSITIVE AND SIMPLE METHOD FOR DETERMINATION OF FERROCYANIDE. Anal Biochem. 1963 Dec;6:549–554. doi: 10.1016/0003-2697(63)90149-0. [DOI] [PubMed] [Google Scholar]
- Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Arnon D. I., Whatley F. R., Allen M. B. Assimilatory Power in Photosynthesis: Photosynthetic phosphorylation by isolated chloroplasts is coupled with TPN reduction. Science. 1958 May 2;127(3305):1026–1034. doi: 10.1126/science.127.3305.1026. [DOI] [PubMed] [Google Scholar]
- Avron M., Chance B. Relation of phosphorylation to electron transport in isolated chloroplasts. Brookhaven Symp Biol. 1966;19:149–160. [PubMed] [Google Scholar]
- Gorman D. S., Levine R. P. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1665–1669. doi: 10.1073/pnas.54.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gorman D. S., Levine R. P. Photosynthetic Electron Transport Chain of Chlamydomonas reinhardi VI. Electron Transport in Mutant Strains Lacking Either Cytochrome 553 or Plastocyanin. Plant Physiol. 1966 Dec;41(10):1648–1656. doi: 10.1104/pp.41.10.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Govindjee R., Thomas J. B., Rabinowitch E. "Second Emerson Effect" in the Hill Reaction of Chlorella Cells with Quinone as Oxidant. Science. 1960 Aug 12;132(3424):421–421. doi: 10.1126/science.132.3424.421. [DOI] [PubMed] [Google Scholar]
- KOK B. Partial purification and determination of oxidation reduction potential of the photosynthetic chlorophyll complex absorbing at 700 millimicrons. Biochim Biophys Acta. 1961 Apr 15;48:527–533. doi: 10.1016/0006-3002(61)90050-6. [DOI] [PubMed] [Google Scholar]
- Kok B., Datko E. A. Reducing power generated in the second photoact of photosynthesis. Plant Physiol. 1965 Nov;40(6):1171–1177. doi: 10.1104/pp.40.6.1171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kok B., Malkin S., Owens O., Forbush B. Observations on the reducing side of the O2-evolving photoact. Brookhaven Symp Biol. 1966;19:446–459. [PubMed] [Google Scholar]
- Smillie R. M. Photosynthetic & respiratory activities of growing pea leaves. Plant Physiol. 1962 Nov;37(6):716–721. doi: 10.1104/pp.37.6.716. [DOI] [PMC free article] [PubMed] [Google Scholar]
