Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1968 Feb;43(2):133–139. doi: 10.1104/pp.43.2.133

Effect of Ozone on Swelling of Tobacco Mitochondria

T T Lee 1
PMCID: PMC1086812  PMID: 16656746

Abstract

The swelling of mitochondria isolated from leaves, roots, and callus tissues of Nicotiana tabacum, L, var. White Gold, was measured by following changes in optical density at 520 mμ in buffered 0.25 m sucrose or 0.125 m KCl. Ozone induced rapid swelling of the isolated mitochondria and increased the permeability of mitochondrial membranes. The extent of mitochondrial swelling and the amount of soluble proteins and other substances absorbing at 260 and 280 mμ released from mitochondria into the suspending medium were positively correlated with the length of exposure to O3. The correlation between the extent of mitochondrial swelling and the loss of intramitochondrial materials was also highly significant.

Protocatechuic acid, when initially present in the suspending medium, completely prevented O3-induced swelling. Reduced glutathione, ascorbate, and l-cysteine partially prevented O3-induced swelling. But these reducing substances failed to reverse mitochondrial swelling when added after O3 treatment.

Polyvinylpyrrolidone (PVP) and bovine serum albumin (BSA) induced contraction of tobacco mitochondria in the absence of O3, but did not reverse swelling of O3-treated mitochondria. PVP and BSA only stopped O3-induced swelling. Evidently O3-induced swelling was osmotically irreversible. Hypertonic solutions of sucrose neither induced contraction of tobacco mitochondria nor prevented O3-induced swelling.

Contraction of tobacco mitochondria occurred rapidly at acid pH, but the contracting state affected by acid pH did not protect mitochondria from O3-induced swelling. Although O3 induced greater swelling at acid pH, the O3-treated mitochondria were still responsive to acid-induced contraction.

Full text

PDF
133

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEYER R. E., ERNSTER L., LOW H., BEYER T. Correlation of optical density and oxidative phosphorylation in reconstructed mitochondrial systems. Exp Cell Res. 1955 Jun;8(3):586–588. doi: 10.1016/0014-4827(55)90140-3. [DOI] [PubMed] [Google Scholar]
  2. BUELL G. C., REISER R. Glyceride-glycerol precursors in the intestinal mucosa. J Biol Chem. 1959 Feb;234(2):217–219. [PubMed] [Google Scholar]
  3. CHAPPELL J. B., GREVILLE G. D. Dependence of mitochondrial swelling on oxidizable substrates. Nature. 1958 Sep 20;182(4638):813–814. doi: 10.1038/182813a0. [DOI] [PubMed] [Google Scholar]
  4. CLELAND K. W. Permeability of isolated rat heart sarcosomes. Nature. 1952 Sep 20;170(4325):497–499. doi: 10.1038/170497a0. [DOI] [PubMed] [Google Scholar]
  5. DAVIES R. E., FONNESU A., PRICE C. A. Movements of water and ions in mitochondria. Biochem J. 1956 Dec;64(4):754–768. doi: 10.1042/bj0640754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HONDA S. I., MUENSTER A. M. Optically-measured and packed volume of lupine mitochondria. Arch Biochem Biophys. 1960 May;88:118–127. doi: 10.1016/0003-9861(60)90206-x. [DOI] [PubMed] [Google Scholar]
  7. Kenefick D. G., Hanson J. B. Contracted state as an energy source for ca binding and ca + inorganic phosphate accumulation by corn mitochondria. Plant Physiol. 1966 Dec;41(10):1601–1609. doi: 10.1104/pp.41.10.1601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEHNINGER A. L., RAY B. L. Oxidation-reduction state of rat liver mitochondria and the action of thyroxine. Biochim Biophys Acta. 1957 Dec;26(3):643–644. doi: 10.1016/0006-3002(57)90115-4. [DOI] [PubMed] [Google Scholar]
  9. LEHNINGER A. L., SCHNEIDER M. Mitochondrial swelling induced by glutathione. J Biophys Biochem Cytol. 1959 Jan 25;5(1):109–116. doi: 10.1083/jcb.5.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. LYONS J. M., PRATT H. AN EFFECT OF ETHYLENE ON SWELLING OF ISOLATED MITOCHONDRIA. Arch Biochem Biophys. 1964 Feb;104:318–324. doi: 10.1016/s0003-9861(64)80020-5. [DOI] [PubMed] [Google Scholar]
  12. Lee T. T. Inhibition of oxidative phosphorylation and respiration by ozone in tobacco mitochondria. Plant Physiol. 1967 May;42(5):691–696. doi: 10.1104/pp.42.5.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lyons J. M., Wheaton T. A., Pratt H. K. Relationship between the Physical Nature of Mitochondrial Membranes and Chilling Sensitivity in Plants. Plant Physiol. 1964 Mar;39(2):262–268. doi: 10.1104/pp.39.2.262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. PACKER L. Metabolic and structural states of mitochondria. I. Regulation by adenosine diphosphate. J Biol Chem. 1960 Jan;235:242–249. [PubMed] [Google Scholar]
  15. SCOTT D. B., LESHER E. C. EFFECT OF OZONE ON SURVIVAL AND PERMEABILITY OF ESCHERICHIA COLI. J Bacteriol. 1963 Mar;85:567–576. doi: 10.1002/path.1700850242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stoner C. D., Hanson J. B. Swelling and contraction of corn mitochondria. Plant Physiol. 1966 Feb;41(2):255–266. doi: 10.1104/pp.41.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TEDESCHI H., HARRIS D. L. The osmotic behavior and permeability to non-electrolytes of mitochondria. Arch Biochem Biophys. 1955 Sep;58(1):52–67. doi: 10.1016/0003-9861(55)90092-8. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES