Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1968 Feb;43(2):229–237. doi: 10.1104/pp.43.2.229

Studies on the Ribonucleic Acid From Wheat Leaves and Chloroplasts 1

D Hadziyev 1,2, S L Mehta 1,3, Saul Zalik 1
PMCID: PMC1086823  PMID: 16656756

Abstract

The chloroplast, leaf ribosomal, and leaf total RNA from seedlings of 2 varieties of Triticum durum and 3 varieties of Triticum vulgare were compared. For a given RNA preparation the major nucleotide composition was the same for all varieties. Irrespective of the variety, the chloroplast total RNA had a higher cytidylic and a lower adenylic acid content than the leaf ribosomal RNA, whereas, the guanylic and uridylic acid content of all RNA preparations was essentially the same. Pseudouridylic acid was present in all RNA preparations and was consistently higher in the durum than the vulgare varieties for leaf ribosomal RNA and leaf total RNA. The leaf ribosomal RNA of all varieties had 2 sub-units with sedimentation coefficients of approximately 18 S and 25 S. The molecular weight of the faster sedimenting subunit was about 2 times that of the slower sedimenting subunit.

Full text

PDF
229

Images in this article

234Image
on p.234

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRAWERMAN G., EISENSTADT J. M. TEMPLATE AND RIBOSOMAL RIBONUCLEIC ACIDS ASSOCIATED WITH THE CHLOROPLASTS AND THE CYTOPLASM OF EUGLENA GRACILIS. J Mol Biol. 1964 Dec;10:403–411. doi: 10.1016/s0022-2836(64)80061-9. [DOI] [PubMed] [Google Scholar]
  2. Bamji M. S., Jagendorf A. T. Amino Acid incorporation by wheat chloroplasts. Plant Physiol. 1966 May;41(5):764–770. doi: 10.1104/pp.41.5.764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CRESTFIELD A. M., ALLEN F. W. Terminal components of ribonucleic acids. Arch Biochem Biophys. 1958 Dec;78(2):334–337. doi: 10.1016/0003-9861(58)90357-6. [DOI] [PubMed] [Google Scholar]
  4. DAVIS F. F., ALLEN F. W. Ribonucleic acids from yeast which contain a fifth nucleotide. J Biol Chem. 1957 Aug;227(2):907–915. [PubMed] [Google Scholar]
  5. GLITZ D. G., DEKKER C. A. THE PURIFICATION AND PROPERTIES OF RIBONUCLEIC ACID FROM WHEAT GERM. Biochemistry. 1963 Nov-Dec;2:1185–1192. doi: 10.1021/bi00906a002. [DOI] [PubMed] [Google Scholar]
  6. Hayes D. H., Hayes F., Guérin M. F. Association of rapidly labelled bacterial RNA with ribosomal RNA in solutions of high ionic strength. II. J Mol Biol. 1966 Jul;18(3):499–515. doi: 10.1016/s0022-2836(66)80039-6. [DOI] [PubMed] [Google Scholar]
  7. Jacobson A. B., Swift H., Bogorad L. Cytochemical studies concerning the occurrence and distribution of RNA in plastids of Zea mays. J Cell Biol. 1963 Jun;17:557–570. doi: 10.1083/jcb.17.3.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KISLEV N., SWIFT H., BOGORAD L. NUCLEIC ACIDS OF CHLOROPLASTS AND MITOCHONDRIA IN SWISS CHARD. J Cell Biol. 1965 May;25:327–344. doi: 10.1083/jcb.25.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. OTAKA E., HOTTA Y., OSAWA S. Occurrence of the fifth nucleotide in soluble ribonucleic acid in yeast. Biochim Biophys Acta. 1959 Sep;35:266–267. doi: 10.1016/0006-3002(59)90364-6. [DOI] [PubMed] [Google Scholar]
  10. Pollard C. J., Stemler A., Blaydes D. F. Ribosomal ribonucleic acids of chloroplastic and mitochondrial preparations. Plant Physiol. 1966 Oct;41(8):1323–1329. doi: 10.1104/pp.41.8.1323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SAGER R., ISHIDA M. R. CHLOROPLAST DNA IN CHLAMYDOMONAS. Proc Natl Acad Sci U S A. 1963 Oct;50:725–730. doi: 10.1073/pnas.50.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SPENCER D., WILDMAN S. G. THE INCORPORATION OF AMINO ACIDS INTO PROTEIN BY CELL-FREE EXTRACTS FROM TOBACCO LEAVES. Biochemistry. 1964 Jul;3:954–959. doi: 10.1021/bi00895a019. [DOI] [PubMed] [Google Scholar]
  13. SPIRIN A. S. The "temperature effect" and macromolecular structure of high-polymer ribonucleic acids of various origin. Biokhimiia. 1961 Nov-Dec;26:454–463. [PubMed] [Google Scholar]
  14. Spencer D. Protein synthesis by isolated spinach chloroplasts. Arch Biochem Biophys. 1965 Aug;111(2):381–390. doi: 10.1016/0003-9861(65)90200-6. [DOI] [PubMed] [Google Scholar]
  15. Spencer D., Whitfeld P. R. The nature of the ribonucleic acid of isolated chloroplasts. Arch Biochem Biophys. 1966 Nov;117(2):337–346. doi: 10.1016/0003-9861(66)90421-8. [DOI] [PubMed] [Google Scholar]
  16. Zscheile F. P., Murray H. C. A Chromatographic-Spectrophotometric Method for Determining Nucleotide Composition of RNA in Plants. Plant Physiol. 1963 Mar;38(2):133–138. doi: 10.1104/pp.38.2.133. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES