Abstract
Chlorella pyrenoidosa Chick (Emerson strain 3) utilizes thiosulfate for growth as effectively as sulfate, and more effectively than a variety of organic sulfur compounds containing sulfur in various oxidation states. Thiosulfates, differentially labeled with 35S in either the SH— or SO3 — sulfur moieties, were used to follow the incorporation of thiosulfate-sulfur into constituents of the insoluble fraction and of the soluble pools. Labeled sulfate was also used for purposes of comparison. Label from both sulfur atoms of thiosulfate and from sulfate is incorporated into the cysteine, homocysteine, and glutathione of the soluble pools, and into the methionine and cystine of protein in the insoluble fraction. Label from SO3-sulfur of thiosulfate is incorporated more slowly into protein methionine and cystine than label from the SH-sulfur. Moreover, the SO3-sulfur of thiosulfate is recovered largely as sulfate in both the soluble pools and the insoluble fraction, while only a trace of SH-sulfur is recovered as sulfate in either case. Consistent with this, the metabolism of the SO3-sulfur of thiosulfate more closely resembles the metabolism of sulfate. Thus it would appear that exogenous thiosulfate undergoes early dismutation in which the SO3-sulfur is preferentially oxidized, and the SH-sulfur is preferentially incorporated in a reduced state. These results are discussed in relation to the conversion of sulfate to thiosulfate by cell-free extracts of Chlorella previously described.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DATTA S. P., DENT C. E., HARRIS H. An apparatus for the simultaneous production of many two-dimensional paper chromatograms. Science. 1950 Nov 24;112(2917):621–623. doi: 10.1126/science.112.2917.621. [DOI] [PubMed] [Google Scholar]
- Hodson R. C., Schiff J. A., Scarsella A. J., Levinthal M. Studies of Sulfate Utilization by Algae. 6. Adenosine-3'-Phosphate-5'-Phosphosulfate (PAPS) as an Intermediate in Thiosulfate Formation From Sulfate by Cell-Free Extracts of Chlorella. Plant Physiol. 1968 Apr;43(4):563–569. doi: 10.1104/pp.43.4.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levinthal M., Schiff J. A. Studies of sulfate utilization by algae. 5. Identification of thiosulfate as a major Acid-volatile product formed by a cell-free sulfate-reducing system from chlorella. Plant Physiol. 1968 Apr;43(4):555–562. doi: 10.1104/pp.43.4.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NAKAMURA T., SATO R. Synthesis from sulphate and accumulation of S-sulphocysteine by a mutant strain of Aspergillus nidulans. Biochem J. 1963 Feb;86:328–335. doi: 10.1042/bj0860328. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SEGEL I. H., JOHNSON M. J. Synthesis and characterization of sodium cysteine-S-sulfate monohydrate. Anal Biochem. 1963 Apr;5:330–337. doi: 10.1016/0003-2697(63)90085-x. [DOI] [PubMed] [Google Scholar]
- Schiff J. A., Levinthal M. Studies of sulfate utilization by algae. 4. Properties of a cell-free sulfate-reducing system from chlorella. Plant Physiol. 1968 Apr;43(4):547–554. doi: 10.1104/pp.43.4.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schiff J. A. Studies of Sulfate Utilization by Algae. II. Further Identification of Reduced Compounds Formed from Sulfate by Chlorella. Plant Physiol. 1964 Mar;39(2):176–179. doi: 10.1104/pp.39.2.176. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schiff J. A. Studies on Sulfate Utilization by Chlorella pyrenoidosa using Sulfate-S; the Occurrence of S-Adenosyl Methionine. Plant Physiol. 1959 Jan;34(1):73–80. doi: 10.1104/pp.34.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]