Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1968 Sep;43(9):1339–1346. doi: 10.1104/pp.43.9.1339

Plant Nucleases. II. Properties of Corn Ribonucleases I and II and Corn Nuclease I

Curtis M Wilson 1,2
PMCID: PMC1087019  PMID: 16656917

Abstract

A classification system is presented to distinguish 3 corn nucleases—Ribonuclease I, Ribonuclease II, and Nuclease I—which were described in the first paper of this series. The 2 ribonucleases are specific for RNA, are endonucleases, and liberate purine and pyrimidine cyclic nucleotides from dinucleotide monophosphates as well as from RNA. Ribonuclease I and II hydrolyze the purine cyclic nucleotides to 3′-nucleotides, while Ribonuclease II may also act on the pyrimidine cyclic nucleotides. Ribonuclease II is best characterized by its molecular weight of 17,000, by a higher pH optimum than Ribonuclease I, and by its absorption onto microsomes.

Nuclease I is a partially purified endonuclease which produces 5′-nucleotides from RNA, DNA, and dinucleotide monophosphates. The same enzyme may also be a 3′-nucleotidase.

The corn nucleases were compared with nucleases found in other plant species.

Full text

PDF
1339

Images in this article

1343Image
on p.1343

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CLARK J. M., Jr, EYZAGUIRRE J. P., GUNTHER J. K. ACTION OF SPLEEN PHOSPHODIESTERASE UPON YEAST SOLUBLE RIBONUCLEIC ACID. J Biol Chem. 1964 Jun;239:1877–1881. [PubMed] [Google Scholar]
  2. FORTI G., TOGNOLI C., PARISI B. Purification from pea leaves of a phosphatase that attacks nucleotides. Biochim Biophys Acta. 1962 Aug 13;62:251–260. doi: 10.1016/0006-3002(62)90038-0. [DOI] [PubMed] [Google Scholar]
  3. Harvey C., Malsman L., Nussbaum A. L. A phosphodiesterase from the carrot. Biochemistry. 1967 Dec;6(12):3689–3694. doi: 10.1021/bi00864a011. [DOI] [PubMed] [Google Scholar]
  4. Ingle J., Hageman R. H. Metabolic changes associated with the germination of corn. II. Nucleic acid metabolism. Plant Physiol. 1965 Jan;40(1):48–53. doi: 10.1104/pp.40.1.48. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. JOYCE B. K., GRISOLIA S. Purification and properties of a nonspecific acid phosphatase from wheat germ. J Biol Chem. 1960 Aug;235:2278–2281. [PubMed] [Google Scholar]
  6. Laskowski M., Sr DNases and their use in the studies of primary structure of nucleic acids. Adv Enzymol Relat Areas Mol Biol. 1967;29:165–220. doi: 10.1002/9780470122747.ch4. [DOI] [PubMed] [Google Scholar]
  7. MEROLA A. J., DAVIS F. F. Preparation and properties of soybean ribonuclease. Biochim Biophys Acta. 1962 Apr 2;55:431–439. doi: 10.1016/0006-3002(62)90976-9. [DOI] [PubMed] [Google Scholar]
  8. REDDI K. K., MAUSER L. J. STUDIES ON THE FORMATION OF TOBACCO MOSAIC VIRUS RIBONUCLEIC ACID. VI. MODE OF DEGRADATION OF HOST RIBONUCLEIC ACID TO RIBONUCLEOSIDES AND THEIR CONVERSION TO RIBONUCLEOSIDE 5'-PHOSPHATES. Proc Natl Acad Sci U S A. 1965 Mar;53:607–613. doi: 10.1073/pnas.53.3.607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Razzell W. E. Plant tissue phosphodiesterase activities. Biochem Biophys Res Commun. 1966 Feb 3;22(3):243–247. doi: 10.1016/0006-291x(66)90472-4. [DOI] [PubMed] [Google Scholar]
  10. SHUSTER L. Ryegrass nucleases. J Biol Chem. 1957 Nov;229(1):289–303. [PubMed] [Google Scholar]
  11. STOCKX J., VANDENDRIESSCHE L., VAN PARIJS R. The hydrolysis of cyclic 2'-3' nucleotides by ammonia during paper chromatography and its influence on quantitative determinations. Arch Int Physiol Biochim. 1962 Feb;70:27–32. doi: 10.3109/13813456209092837. [DOI] [PubMed] [Google Scholar]
  12. SUNG S. C., LASKOWSKI M., Sr A nuclease from mung bean sprouts. J Biol Chem. 1962 Feb;237:506–511. [PubMed] [Google Scholar]
  13. Stockx J., Dierick W., Vandendriessche L. About a phosphodiesterase in Phaseolus aureus Roxb. Arch Int Physiol Biochim. 1965 Jun;73(3):421–431. doi: 10.3109/13813456509081853. [DOI] [PubMed] [Google Scholar]
  14. TUVE T. W., ANFINSEN C. B. Preparation and properties of spinach ribonuclease. J Biol Chem. 1960 Dec;235:3437–3441. [PubMed] [Google Scholar]
  15. Walters T. L., Loring H. S. Enzymes of nucleic acid metabolism from mung bean sprouts. I. Fractionation and concentration of phosphomonoesterase, ribonucleases M1 and M2, 3'-nucleotidase, and deoxyribonuclease. J Biol Chem. 1966 Jun 25;241(12):2870–2875. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES