Abstract
Red beet root discs aerated in potassium phosphate for 2 to 3 days and young spinach leaves actively produce oxalate. A series of labeled compounds was supplied to each of these tissues to determine the extent of conversion to oxalate. Similar results were obtained with the 2 tissues except that in the leaf tissue glyoxylate and glycolate were outstandingly good precursors. Carbon from glucose, acetate, and particularly from some acids of the tricarboxylic acid cycle was recovered in oxalate. Extracts from both tissues were found to contain an enzyme which converts oxaloacetate to oxalate and acetate. The enzyme was partially purified and some of its properties are described. A pathway of oxalate synthesis which does not include glycolate or its oxidase is therefore proposed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Giovanelli J. Oxalyl-coenzyme A synthetase from pea seeds. Biochim Biophys Acta. 1966 Apr 12;118(1):124–143. doi: 10.1016/s0926-6593(66)80151-0. [DOI] [PubMed] [Google Scholar]
- Millerd A., Morton R. K., Wells J. R. Oxalic acid synthesis in shoots of Oxalis pes-caprae. The precursors of glycollic acid and glyoxylic acid. Biochem J. 1963 Aug;88(2):276–281. doi: 10.1042/bj0880276. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RICHARDSON K. E., TOLBERT N. E. Oxidation of glyoxylic acid to oxalic acid by glycolic acid oxidase. J Biol Chem. 1961 May;236:1280–1284. [PubMed] [Google Scholar]