Abstract
Chloroplast RNA is synthesized in dark-grown radish cotyledons at about one-third the rate of that in the light. The synthesis, however, continues for longer in the dark and the percentage of chloroplast RNA can approach that in light-grown tissue. Light stimulates the synthesis and accumulation of both cytoplasmic and chloroplast RNA, but shows a 4-fold greater stimulation of the chloroplast RNA. Chloramphenicol, streptomycin and cycloheximide inhibit the synthesis of chloroplast RNA with little effect on cytoplasmic RNA. 5-Fluorouracil inhibits the synthesis of cytoplasmic more than chloroplast RNA. Synthesis of the 0.56 × 106 mol wt chloroplast RNA is inhibited much less than the other ribosomal RNA components by actinomycin D.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bartels P. G., Matsuda K., Siegel A., Weier T. E. Chloroplastic ribosome formation: inhibition by 3-amino-1,2,4-triazole. Plant Physiol. 1967 May;42(5):736–741. doi: 10.1104/pp.42.5.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boardman N. K. Ribosome composition and chloroplast development in Phaseolus vulgaris. Exp Cell Res. 1966 Sep;43(2):474–482. doi: 10.1016/0014-4827(66)90074-7. [DOI] [PubMed] [Google Scholar]
- Loening U. E., Ingle J. Diversity of RNA components in green plant tissues. Nature. 1967 Jul 22;215(5099):363–367. doi: 10.1038/215363a0. [DOI] [PubMed] [Google Scholar]
- Ritenour G. L., Joy K. W., Bunning J., Hageman R. H. Intracellular localization of nitrate reductase, nitrite reductase, and glutamic Acid dehydrogenase in green leaf tissue. Plant Physiol. 1967 Feb;42(2):233–237. doi: 10.1104/pp.42.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smillie R. M., Graham D., Dwyer M. R., Grieve A., Tobin N. F. Evidence for the synthesis in vivo of proteins of the Calvin cycle and of the photosynthetic electron-transfer pathway on chloroplast ribosomes. Biochem Biophys Res Commun. 1967 Aug 23;28(4):604–610. doi: 10.1016/0006-291x(67)90356-7. [DOI] [PubMed] [Google Scholar]