Fig. 7. CD4-Ig variants bind Env trimers with higher affinity than CD4-Ig-v0.
a Fitted sensorgrams show one of two replicates of the indicated CD4-Ig variant binding to 16055-ConM-v8.1 SOSIP trimers. Anti-human IgG antibodies were immobilized on the surface to capture CD4-Ig. After CD4-Ig capture, SOSIP protein was injected at concentrations of 800, 400, 200, 100, 50 nM in single-cycle kinetics at 25°C. The experimental data were fitted with a 1:1 Langmuir model. b Summary of Koff, Kon and Kd for CD4-Ig variants. Kd is calculated from Koff and Kon.