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. Author manuscript; available in PMC: 2024 Feb 19.
Published in final edited form as: Cell. 2023 Mar 20;186(7):1398–1416.e23. doi: 10.1016/j.cell.2023.02.027

Figure 6. Edited CD3δ SCID ATO-derived T cells Express Features of Maturation without Evidence of Exhaustion.

Figure 6.

a) Representative flow cytometry profiles depicting maturation markers (CCR7, CD62L, CD27, CD28, CD45RO, and CD45RA) in cells gated on SP8 T cells - CD3+TCRαβ+CD8α+CD8β+, in week 12 ATOs (n=9, from four independent experiments). b) RNA expression of selected genes (y-axis) across clusters in edited patient ATOs by CITE-seq; Cyt., cytokine. c) Gene Set Enrichment Analysis (GSEA) of differentially expressed genes from GOBP (Gene Ontology Biological Process) and GOCC (Gene Ontology Cellular Compartment) between SP8 T and DN cells from edited ATOs. Dot size represents adjusted p-value (padj; two-sided permutation test). NES, normalized enrichment score; PM, plasma membrane. GSEA plots of representative gene sets d) alpha beta T cell differentiation (p=0.035), and e) TCR complex (p=1.74E-8) in SP8 vs DN T cells from edited ATOs. f) Representative flow cytometry profiles of exhaustion markers in SP8 T cells directly from week 15 HD (n=9) and edited patient ATOs (n=9), and compared to PBMCs (n=3); PBMC were stimulated with (orange) and without (purple) anti-CD3/28 beads and IL2 for 24 hours.