Correction to “Analysis of the Sequence Preference of Saporin by Deep Sequencing”

“RNA Loop” library data in Figure 5B were inadvertently replaced with those of the “RNA SRL” library data presented in the same panel. The corrected Figure 5 is provided below.

Figure 5.

Distribution of recovery ratios. (A) Shape of the distribution of recovery ratios for all variants. The distributions of the SRL-based libraries are flatter than those of the loop libraries indicative of a more promiscuous activity. (B) Dependence of recovery on the number of adenine residues. An increasing number of adenine residues reduces the recovery ratios of variants, indicating that adenine is targeted nonspecifically. Interestingly, there are some variants in both DNA libraries that exhibit much lower recovery ratios compared to those of the rest of the population. This indicates that some specific sequence pattern is targeted. (C) Minimum free energy structures of two sensitive variants from the SRL DNA library as predicted by NUPACK.³³ These variants form secondary hairpins with small loops. The small loops closely resemble the sensitive ACG pattern identified in the DNA loop library.

“RNA Loop” library data in Figure S4 in the Supporting Information were inadvertently replaced with those of the “RNA SRL” library data presented in the same panel. A new Supporting Information file with the corrected figure is provided with this correction.

Supporting Information Available

The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acschembio.3c00733.

• CR protocols, native PAGE experiments that confirm the oligos used do not form duplexes, control experiments for the nonspecific depurination of DNA oligos, linear correlations between two replicates for all libraries and conditions, correlations of the recovery ratio to the number of each nucleotide base, and a picture of the second replicate of the PAGE gel shown in Figure 1 (PDF)