Fig. 4.

Representative confocal laser scanning microscopy images showing dual immunofluorescent staining for GLUT1 protein (red) and nuclear counterstain (blue) in PHT at 90 h. PHT were treated for 24 h with 50 μmol/L glyburide (A) or were untreated (B). 1 μm step interval Z-stack images were captured at 63× (A–B); scale bar 50 μm. Arrows indicate GLUT1 staining associated with PHT cell surface (A).