Table 1.
Dilutions | Manual Method (Reference One) | Automated Method (Evaluated One) | ||||||
---|---|---|---|---|---|---|---|---|
A (ND) | B (10−1) | C (10−2) | D (10−3) | A (ND) | B (10−1) | C (10−2) | D (10−3) | |
Rep. #1 | 1.00 × 109 | 1.00 × 108 | 7.33 × 106 | 1.53 × 106 | 1.07 × 109 | 1.33 × 108 | 1.33 × 107 | 1.13 × 106 |
Rep. #2 | 7.33 × 108 | 1.20 × 108 | 1.40 × 107 | 1.73 × 106 | 8.67 × 108 | 1.20 × 108 | 1.07 × 107 | 1.13 × 106 |
Rep. #3 | 1.53 × 109 | 1.07 × 108 | 1.07 × 107 | 2.07 × 106 | 1.40 × 109 | 1.47 × 108 | 1.27 × 107 | 1.00 × 106 |
Rep. #4 | 8.67 × 108 | 1.47 × 108 | 1.00 × 107 | 1.27 × 106 | 1.00 × 109 | 1.20 × 108 | 1.33 × 107 | 8.67 × 105 |
Mean of Rep. | 1.03 × 109 | 1.18 × 108 | 1.05 × 107 | 1.65 × 106 | 1.08 × 109 | 1.30 × 108 | 1.25 × 107 | 1.03 × 106 |
SD | 3.51 × 108 | 2.06 × 107 | 2.74 × 106 | 3.37 × 105 | 2.27 × 108 | 1.28 × 107 | 1.26 × 106 | 1.28 × 105 |
CV (%) | 33.93 | 17.44 | 26.11 | 20.44 | 20.94 | 9.82 | 10.10 | 12.35 |
Mean CV (%) | 24.48 (±7.25) | 13.30 (±5.21) |
ND: non-diluted, Rep.: independent replicate, SD: standard deviation, and CV: coefficient of variation.