Figure 1. In vitro fungicidal activity of miltefosine and milteforan against the yeast morphology of S. schenckii and S. brasiliensis.

a) S. schenckii (strains 4820, 4821, and 4822) yeast were grown in liquid YDP pH 7.8 at 37°C in the presence of several concentrations of MFS or ML (16, 8, 4, 2, 1, 0.5, and 0.25μg/mL). After 4 days of incubation, the cells were plated in solid YPD pH 7.8 and incubated for 4 days at 37°C. b) S. brasiliensis (strains 4823, 4824, and 4858) yeast were grown in liquid YDP pH 7.8 at 37°C in the presence of several concentrations of MFS or ML (16, 8, 4, 2, 1, 0.5, and 0.25μg/mL). After 4 days of incubation, the cells were plated in solid YPD pH 7.8 and incubated for 4 days at 37°C. As control, yeast cells of each strain were grown without the drugs. Results represent the average of three independent experiments performed by duplicate.