Table:

Patient demographics and qPCR Ct. values of gemykibivirus positive cases

Sample Id†	Specimen	Age group [yrs]	Gender	Ct. Gemy_1‡	Ct. Gemy_2§	Specimen collection date	District¶	Province
N0000218	CSF	11–15	M	32.42	30.68	2020 Feb	Kaski	Gandaki
N0000256	CSF	0–5	F	29.41	29.34	2020 July	Myagdi	Gandaki
N0000300	CSF	6–10	F	32.95	31.46	2020 Sep	Palpa	Lumbini
N0000358	Serum	0–5	M	29.27	29.94	2022 Feb	Sarlahi	Madhesh
N0000359	CSF	0–5	F	27.4	27.08	2022 Feb	Kapilvastu	Lumbini
N0000395	CSF	0–5	M	30.74	30.73	2022 April	Kaski	Gandaki
N0000434	CSF	56–60	M	29.02	30.06	2022 June	Lalitpur	Bagmati
N0000468	CSF	30–35	F	30.84	31.47	2022 July	Chitwan	Bagmati
N0000490	CSF	unknown	M	29.86	32.08	2022 July	Syangja	Gandaki
N0000545	CSF	70–75	F	26.84	25.35	2022 Aug	Kathmandu	Bagmati
N0000546	CSF	20–25	M	24.65	23.53	2022 Aug	Kathmandu	Bagmati
N0000722	CSF	20–25	F	28.74	29.72	2022 Nov	Chitwan	Bagmati

^*Ct., cycle threshold; yrs, years.

^†The sample IDs are coded and are not known outside the research group.

^‡Gemy_1: qPCR targeting capsid protein gene.

^§Gemy_2: qPCR targeting replication associated protein (REP) gene.

^¶Districts involved are highlighted in Figure 3 as shade.