Fig. 5.

H₂O₂- and NO-induced activation of MN death. MNs were isolated from FUS^R521C mice (week 18). For the analysis of cell death (a) and the tests related to the molecular activation of apoptosis (b), cells were treated as indicated in Table 2. Data are means ​± ​SD of 4-5 different experiments. (c) Mitophagy analyses using lysosomal- and mitochondrial specific fluorescent dyes in live MNs. The graph corresponds to the average percentage of lysotracker puncta that colocalize with mitochondria (mean values ​± ​SD for 4–5 different mice per experimental condition). A one-way analysis of variance (ANOVA) was used to make comparisons among the different treatments. Different letters indicate statistical differences, P ​< ​0.05.