Figure 1.

Genetic engineering of novel NSG mice via CRISPR

(A) Schematic representation of CRISPR/Cas9 at mouse Flt3 locus and the chromosomal deletion at the exon 3. Green lines represent the mouse sequences and orange lines represent the mutated sequences. Black boxes represent the coding region and white box represent the non-coding region. gRNAs are labeled with red arrowhead and the primer pairs F/R are in gray.

(B) The summary of percentage (upper) and total number (lower) of mouse pDCs and cDCs from bone marrow, spleen and lungs of 8–10 weeks of mice (mean ± SD, n = 7).

(C) Mouse Flt3L in the plasma of 8–10 weeks old mice were analyzed by ELISA (mean, n = 7).

(D) Schematic representation of CRISPR/Cas9 at mouse Il6 locus, the vector for human IL6 donor DNA and targeted allele with homologous recombination. Green lines represent the mouse sequences and orange lines represent the human IL6 sequences. Black boxes represent the coding region and white box represent the non-coding region. gRNAs are labeled with red arrowhead. Blue boxes represent the left and right homologous arms. One of the primer pairs F1/R1 and F2/R2 in gray is located outside of the homologous arms, respectively.

(E) Human IL-6 production (mean, n = 3–6) in the plasma of mice collected at 2 h after i.p. treatment of LPS (10 μg).

(F) Human IL-6 production (mean, n = 3) in the plasma of mice collected at 2 h after i.p. treatment of LPS (0.1, 0.5, 2.5, or 10 μg).

See also Figure S1.