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. 1996 Nov;51(11):1123–1126. doi: 10.1136/thx.51.11.1123

Gamma/delta cells in tissue from patients with sarcoidosis.

M L Wilsher 1, M Hallowes 1, N M Birchall 1
PMCID: PMC1090524  PMID: 8958896

Abstract

BACKGROUND: Because gamma/delta T lymphocytes (gamma delta cells) respond to myco-bacterial antigens in vitro and accumulate in the skin lesions of patients with certain granulomatous infections (leprosy, leishmaniasis), it was hypothesised that these cells might have a role in the pathogenesis of sarcoidosis, a disease also characterised by granuloma formation. Having failed to demonstrate an increase in gamma delta cells in the blood of patients with sarcoidosis, the aim of this study was to examine samples of bronchoalveolar lavage (BAL) fluid and biopsy tissue. METHODS: Samples from 23 patients (13 women) with newly diagnosed sarcoidosis, of mean age 31 years and median percentage of lymphocytes in the BAL fluid of 31%, were studied. Controls included normal subjects and patients with other interstitial lung diseases (ILD). Cytopreparations of BAL fluid (n = 13) and cryostat sections (five mediastinal nodes, 14 transbronchial biopsies) were stained with alkaline phosphatase-antialkaline phosphatase and monoclonal antibodies to CD3, CD4, CD8, CD25, and gamma delta T cell receptor (TCR). RESULTS: All patients had typical chest radiographs (16 stage I, four stage II, three stage III). All were Mantoux negative with negative tuberculosis cultures. Compared with normal controls and patients with other interstitial lung diseases there was no increase in gamma delta cells in the BAL fluid (sarcoidosis, 1% (range 0-4%) total cells; ILD, 1% (0-2%); controls, 0.5% (0-2%); p > 0.05, Kruskal-Wallis). Likewise, there was no increase in gamma delta cells in the transbronchial biopsy specimens (sarcoidosis, 1/high power field (hpf) (range 0-2); ILD, < 1/hpf (0-4); controls < 1/hpf (0-2); p > 0.05). gamma delta cells were rarely seen in the lymph nodes in spite of the presence of numerous granulomas. CONCLUSION: These results provide further evidence that gamma delta cells are not increased in most patients with sarcoidosis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asarnow D. M., Kuziel W. A., Bonyhadi M., Tigelaar R. E., Tucker P. W., Allison J. P. Limited diversity of gamma delta antigen receptor genes of Thy-1+ dendritic epidermal cells. Cell. 1988 Dec 2;55(5):837–847. doi: 10.1016/0092-8674(88)90139-0. [DOI] [PubMed] [Google Scholar]
  2. Augustin A., Kubo R. T., Sim G. K. Resident pulmonary lymphocytes expressing the gamma/delta T-cell receptor. Nature. 1989 Jul 20;340(6230):239–241. doi: 10.1038/340239a0. [DOI] [PubMed] [Google Scholar]
  3. Balbi B., Valle M. T., Oddera S., Giunti D., Manca F., Rossi G. A., Allegra L. T-lymphocytes with gamma delta+ V delta 2+ antigen receptors are present in increased proportions in a fraction of patients with tuberculosis or with sarcoidosis. Am Rev Respir Dis. 1993 Dec;148(6 Pt 1):1685–1690. doi: 10.1164/ajrccm/148.6_Pt_1.1685. [DOI] [PubMed] [Google Scholar]
  4. Bluestone J. A., Matis L. A. TCR gamma delta cells--minor redundant T cell subset or specialized immune system component? J Immunol. 1989 Mar 15;142(6):1785–1788. [PubMed] [Google Scholar]
  5. Born W., Happ M. P., Dallas A., Reardon C., Kubo R., Shinnick T., Brennan P., O'Brien R. Recognition of heat shock proteins and gamma delta cell function. Immunol Today. 1990 Feb;11(2):40–43. doi: 10.1016/0167-5699(90)90015-2. [DOI] [PubMed] [Google Scholar]
  6. Du Bois R. M., Kirby M., Balbi B., Saltini C., Crystal R. G. T-lymphocytes that accumulate in the lung in sarcoidosis have evidence of recent stimulation of the T-cell antigen receptor. Am Rev Respir Dis. 1992 May;145(5):1205–1211. doi: 10.1164/ajrccm/145.5.1205. [DOI] [PubMed] [Google Scholar]
  7. Forrester J. M., Newman L. S., Wang Y., King T. E., Jr, Kotzin B. L. Clonal expansion of lung V delta 1+ T cells in pulmonary sarcoidosis. J Clin Invest. 1993 Jan;91(1):292–300. doi: 10.1172/JCI116184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grunewald J., Shigematsu M., Nagai S., Mikuniya T., Wigzell H., Izumi T., Eklund A. G. T-cell receptor V gene expression in HLA-typed Japanese patients with pulmonary sarcoidosis. Am J Respir Crit Care Med. 1995 Jan;151(1):151–156. doi: 10.1164/ajrccm.151.1.7812546. [DOI] [PubMed] [Google Scholar]
  9. Ito M., Kojiro N., Ikeda T., Ito T., Funada J., Kokubu T. Increased proportions of peripheral blood gamma delta T cells in patients with pulmonary tuberculosis. Chest. 1992 Jul;102(1):195–197. doi: 10.1378/chest.102.1.195. [DOI] [PubMed] [Google Scholar]
  10. Modlin R. L., Pirmez C., Hofman F. M., Torigian V., Uyemura K., Rea T. H., Bloom B. R., Brenner M. B. Lymphocytes bearing antigen-specific gamma delta T-cell receptors accumulate in human infectious disease lesions. Nature. 1989 Jun 15;339(6225):544–548. doi: 10.1038/339544a0. [DOI] [PubMed] [Google Scholar]
  11. Nakata K., Sugie T., Nakano H., Sakai T., Aoki M. Gamma-delta T cells in sarcoidosis. Correlation with clinical features. Am J Respir Crit Care Med. 1994 Apr;149(4 Pt 1):981–988. doi: 10.1164/ajrccm.149.4.8143064. [DOI] [PubMed] [Google Scholar]
  12. O'Brien R. L., Happ M. P., Dallas A., Palmer E., Kubo R., Born W. K. Stimulation of a major subset of lymphocytes expressing T cell receptor gamma delta by an antigen derived from Mycobacterium tuberculosis. Cell. 1989 May 19;57(4):667–674. doi: 10.1016/0092-8674(89)90135-9. [DOI] [PubMed] [Google Scholar]
  13. Raulf M., Liebers V., Steppert C., Baur X. Increased gamma/delta-positive T-cells in blood and bronchoalveolar lavage of patients with sarcoidosis and hypersensitivity pneumonitis. Eur Respir J. 1994 Jan;7(1):140–147. doi: 10.1183/09031936.94.07010140. [DOI] [PubMed] [Google Scholar]
  14. Takagaki Y., DeCloux A., Bonneville M., Tonegawa S. Diversity of gamma delta T-cell receptors on murine intestinal intra-epithelial lymphocytes. Nature. 1989 Jun 29;339(6227):712–714. doi: 10.1038/339712a0. [DOI] [PubMed] [Google Scholar]
  15. Tamura N., Holroyd K. J., Banks T., Kirby M., Okayama H., Crystal R. G. Diversity in junctional sequences associated with the common human V gamma 9 and V delta 2 gene segments in normal blood and lung compared with the limited diversity in a granulomatous disease. J Exp Med. 1990 Jul 1;172(1):169–181. doi: 10.1084/jem.172.1.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tamura N., Moller D. R., Balbi B., Crystal R. G. Preferential usage of the T-cell antigen receptor beta-chain constant region C beta 1 element by lung T-lymphocytes of patients with pulmonary sarcoidosis. Am Rev Respir Dis. 1991 Mar;143(3):635–639. doi: 10.1164/ajrccm/143.3.635. [DOI] [PubMed] [Google Scholar]
  17. Tazi A., Bouchonnet F., Valeyre D., Cadranel J., Battesti J. P., Hance A. J. Characterization of gamma/delta T-lymphocytes in the peripheral blood of patients with active tuberculosis. A comparison with normal subjects and patients with sarcoidosis. Am Rev Respir Dis. 1992 Nov;146(5 Pt 1):1216–1221. doi: 10.1164/ajrccm/146.5_Pt_1.1216. [DOI] [PubMed] [Google Scholar]
  18. Tazi A., Fajac I., Soler P., Valeyre D., Battesti J. P., Hance A. J. Gamma/delta T-lymphocytes are not increased in number in granulomatous lesions of patients with tuberculosis or sarcoidosis. Am Rev Respir Dis. 1991 Dec;144(6):1373–1375. doi: 10.1164/ajrccm/144.6.1373. [DOI] [PubMed] [Google Scholar]
  19. Wilsher M. L., Hallowes M., Birchall N. M. Gamma/delta T lymphocytes in the blood of patients with sarcoidosis. Thorax. 1995 Aug;50(8):858–862. doi: 10.1136/thx.50.8.858. [DOI] [PMC free article] [PubMed] [Google Scholar]

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