Fig. 5.

LMO7-mediated bioluminescence-driven excitation of SNr neurons elicits motor behavioral changes. (a) Schematic illustration of the expression strategy for unilateral photoactivation of substantia nigra pars reticulata (SNr) neurons. AAV-hSyn LMO7 was injected in SNr of wild type mice. (b) Instantaneous time lapsed trajectory of the snout of a representative mouse in an open field treated with either vehicle (left) or FFz (right). (c) Schematic illustration of body parts (snout and base of the tail) extracted with DeepLabCut between consecutive video frames for instantaneous rotational analysis. (d) Summary data showing the cumulative rotation degrees in mice expressing LMO7 during a 160-s-long episode treated with vehicle or FFz. Inset, calculation of rotation degree ($\theta$) for each frame (40 ms bins). $n=3$ animals. Data are presented as mean ± SEM. (e) Summary data showing the total degrees rotated in each experimental condition; $p=0.04$. (f) Turning preference displayed as the ratio of ipsilateral over contralateral revolutions during a 10 min duration; $p=0.01$. (g) Total number of grooming bouts ($p=0.06$) and (h) total time spent grooming ($p=0.006$) between vehicle and FFz treated mice. * $p<0.05$, ** $p<0.01$.