Extended Data Fig. 4. Effects of TGN020 on CSF tracer dynamics and behavior.
a. Experimental schematic. C57BL6/J mice received intraperitoneal injection of TGN020 or DMSO control. CSF tracer was infused via intracisternal magna and tracer was allowed to circulate for 60 min. b. Example images of CSF tracer (FITC-3kD-dextran) infused to cisterna magna and quantified in frontal cortex. Scale bar, 30 μm. c. Quantification of CSF tracer signal in frontal cortex (n = 5 C57BL6/J mice per group; data presented as mean +/− SEM; P value was calculated by unpaired two-tailed student’s t-test). d. Experimental schematic. 6-month-old 5XFAD mice were subjected to daily (1 h/day) of audio + visual 40 Hz stimulation (control = white noise and random flicker at the same lux and sound intensity). Mice received i.p. injections of vehicle or TGN020 prior to daily stimulation. e. Novel object recognition testing was performed on days 10 and 11. f. Quantification of discrimination index following novel object recognition test quantified as percent of time spent exploring novel object (n = 13 6-month-old 5XFAD mice for Vehicle + No Stim, 14 animals for Vehicle + 40 Hz; 13 mice for TGN020 + No Stim; 13 mice for TGN020 + 40 Hz. P values represent two-way ANOVA followed by Fisher’s LSD multiple comparison’s test; data is presented as the mean ± s.e.m.).