Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1978 Jan;61(1):80–84. doi: 10.1104/pp.61.1.80

Chlorophyll Fluorescence Assay for Ozone Injury in Intact Plants 1

Ulrich Schreiber 1,2,3, William Vidaver 1,2,3, Victor C Runeckles 1,2,3, Peter Rosen 1,2,3
PMCID: PMC1091801  PMID: 16660243

Abstract

A chlorophyll fluorescence induction (Kautsky effect) assay predicted ozone-induced injury in bean leaves (Phaseolus vulgaris) at least 20 hours before any visible sign of leaf necrosis. The extent of injury, which could be predicted during exposure to ozone, depended on concentration, exposure time, and leaf development stage. Much more injury occurred in light than in darkness and long exposures to lower ozone concentrations were more injurious than brief exposures to higher ones. The first detectable effect was on the photosynthetic water-splitting enzyme systems, followed by inhibition of electron transport between the photosystems. The fluorescence assay provides a simple, rapid, nondestructive method for observing effects of ozone on plants.

Full text

PDF
80

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chimiklis P. E., Heath R. L. Ozone-induced Loss of Intracellular Potassium Ion from Chlorella sorokiniana. Plant Physiol. 1975 Dec;56(6):723–727. doi: 10.1104/pp.56.6.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coulson C., Heath R. L. Inhibition of the photosynthetic capacity of isolated chloroplasts by ozone. Plant Physiol. 1974 Jan;53(1):32–38. doi: 10.1104/pp.53.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Heck W. W., Dunning J. A., Hindawi I. J. Ozone: nonlinear relation of dose and injury in plants. Science. 1966 Feb 4;151(3710):577–578. doi: 10.1126/science.151.3710.577. [DOI] [PubMed] [Google Scholar]
  4. Krause G. H. The high-energy state of the thylakoid system as indicated by chlorophyll fluorescence and chloroplast shrinkage. Biochim Biophys Acta. 1973 Apr 5;292(3):715–728. doi: 10.1016/0005-2728(73)90019-4. [DOI] [PubMed] [Google Scholar]
  5. Murata N., Sugahara K. Control of excitation transfer in photosynthesis. 3. Light-induced decrease of chlorophyll a fluorescence related to photophosphorylation system in spinach chloroplasts. Biochim Biophys Acta. 1969 Oct 21;189(2):182–192. doi: 10.1016/0005-2728(69)90046-2. [DOI] [PubMed] [Google Scholar]
  6. Pell E. J., Brennan E. Changes in respiration, photosynthesis, adenosine 5'-triphosphate, and total adenylate content of ozonated pinto bean foliage as they relate to symptom expression. Plant Physiol. 1973 Feb;51(2):378–381. doi: 10.1104/pp.51.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Schreiber U., Vidaver W. Chlorophyll fluorescence induction in anaerobic Scenedesmus obliquus. Biochim Biophys Acta. 1974 Oct 18;368(1):97–112. doi: 10.1016/0005-2728(74)90100-5. [DOI] [PubMed] [Google Scholar]
  8. Schreiber U., Vidaver W. Hydrostatic pressure: a reversible inhibitor of primary photosynthetic processes. Z Naturforsch C. 1973 Nov-Dec;28(11):704–709. doi: 10.1515/znc-1973-11-1210. [DOI] [PubMed] [Google Scholar]
  9. Wraight C. A., Crofts A. R. Energy-dependent quenching of chlorophyll alpha fluorescence in isolated chloroplasts. Eur J Biochem. 1970 Dec;17(2):319–327. doi: 10.1111/j.1432-1033.1970.tb01169.x. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES