Abstract
Wounding of potato (Solanum tuberosum L.) tubers results in suberization, apparently triggered by the release of some chemical factor(s) at the cut surface. Suberization, as measured by diffusion resistance of the tissue surface to water vapor, was inhibited by mm concentrations of indoleacetic acid, unaffected by mm concentrations of traumatic acid, severely inhibited at μm concentrations of cytokinin, but stimulated by abscisic acid (ABA) at 10−4 m. Thorough washing of potato disks up to 3 to 4 days after cutting resulted in severe inhibition of suberization as measured both by diffusion resistance and by the amount of the octadecene diol generated by hydrogenolysis (LiAlH4) of the tissue. Disks washed after 4 days did not show any inhibition of suberization. High performance liquid chromatographic analysis of the wash from fresh potato disks showed that about 14 ng of ABA was released into the wash per g of tissue. The amount of ABA released increased with time up to 4 to 6 hours of washing. The maximal amount of ABA was washed out after aging for 24 hours and after 2 days of aging ABA could no longer be found in the surface wash of the disks. Addition of ABA to the media of potato tissue cultures resulted in suberin formation whereas control cultures contained little suberin. The effect of ABA on suberization in the tissue cultures was shown to be linearly concentration-dependent up to 10−4 m and a linear increase in suberin formation was seen up to about 8 days of culture growth on the media containing 10−4 m ABA. From these results it is proposed that during the early phase of wound-healing ABA plays a role in triggering a chain of biochemical processes which eventually (in about 3 to 4 days) result in the formation of a suberization-inducing factor, responsible for the induction of the enzymes involved in suberin biosynthesis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Agrawal V. P., Kolattukudy P. E. Biochemistry of Suberization: omega-Hydroxyacid Oxidation in Enzyme Preparations from Suberizing Potato Tuber Disks. Plant Physiol. 1977 Apr;59(4):667–672. doi: 10.1104/pp.59.4.667. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dean B. B., Kolattukudy P. E. Biochemistry of Suberization: Incorporation of [1-C]Oleic Acid and [1-C]Acetate into the Aliphatic Components of Suberin in Potato Tuber Disks (Solanum tuberosum). Plant Physiol. 1977 Jan;59(1):48–54. doi: 10.1104/pp.59.1.48. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dean B. B., Kolattukudy P. E. Synthesis of Suberin during Wound-healing in Jade Leaves, Tomato Fruit, and Bean Pods. Plant Physiol. 1976 Sep;58(3):411–416. doi: 10.1104/pp.58.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kolattukudy P. E., Dean B. B. Structure, gas chromatographic measurement, and function of suberin synthesized by potato tuber tissue slices. Plant Physiol. 1974 Jul;54(1):116–121. doi: 10.1104/pp.54.1.116. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kolattukudy P. E., Kronman K., Poulose A. J. Determination of structure and composition of suberin from the roots of carrot, parsnip, rutabaga, turnip, red beet, and sweet potato by combined gas-liquid chromatography and mass spectrometry. Plant Physiol. 1975 Mar;55(3):567–573. doi: 10.1104/pp.55.3.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sweetser P. B., Vatvars A. High-performance liquid chromatographic analysis of abscisic acid in plant extracts. Anal Biochem. 1976 Mar;71(1):68–78. doi: 10.1016/0003-2697(76)90012-9. [DOI] [PubMed] [Google Scholar]
- Walton T. J., Kolattukudy P. E. Determination of the structures of cutin monomers by a novel depolymerization procedure and combined gas chromatography and mass spectrometry. Biochemistry. 1972 May 9;11(10):1885–1896. doi: 10.1021/bi00760a025. [DOI] [PubMed] [Google Scholar]