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. 1978 Feb;61(2):254–258. doi: 10.1104/pp.61.2.254

Indole-3-acetic Acid Levels of Plant Tissue as Determined by a New High Performance Liquid Chromatographic Method 1

Philip B Sweetser 1, Dennis G Swartzfager 1
PMCID: PMC1091843  PMID: 16660271

Abstract

A method for the analysis of indole-3-acetic acid (IAA) in plant extracts has been developed based on high performance liquid chromatography separation of IAA on a microparticulate strong anion exchange column followed by quantitation with two selective detectors: an electrochemical, carbon paste amperometric detector and/or a fluorescence detector. The detection limit for IAA is less than 1 nanogram with the fluorescence detector and less than 50 picograms with the electrochemical detector.

The IAA levels are reported for various tissues of wheat, pinto beans, soybeans, cotton, and corn.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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