Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1978 Feb;61(2):274–277. doi: 10.1104/pp.61.2.274

Evidence That the Lipid Carrier for N-Acetylglucosamine Is Different from That for Mannose in Mung Beans and Cotton Fibers 1

Mary C Ericson 1, John T Gafford 1, Alan D Elbein 1
PMCID: PMC1091847  PMID: 16660275

Abstract

Cell-free enzyme particles from mung beans (Phaseolus aureus) or cotton (Gossypium hirsutum L.) fibers catalyze the incorporation of mannose from GDP-[14C]mannose and N-acetylglucosamine from UDP-[3H]-N-acetylglucosamine into polyprenyl-type lipids. These lipids have been synthesized and purified and the lipid moieties compared to each other as well as to dolichyl phosphate and to lipids isolated from similar mannoseand N-acetylglucosamine-containing lipids from liver and aorta.

The following lines of evidence indicate that in plants, the lipid carrier for N-acetylglucosamine is different from the lipid carrier for mannose: [List: see text]

We propose that the apparent difference in the lipid carrier for these two sugars may be a point of control of glycoprotein synthesis.

Full text

PDF
274

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brett C. T., Leloir L. F. Dolichyl monophosphate and its sugar derivatives in plants. Biochem J. 1977 Jan 1;161(1):93–101. doi: 10.1042/bj1610093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chambers J., Forsee W. T., Elbein A. D. Enzymatic transfer of mannose from mannosyl-phosphoryl-polyprenol to lipid-linked oligosaccharides by pig aorta. J Biol Chem. 1977 Apr 25;252(8):2498–2506. [PubMed] [Google Scholar]
  3. Chen W. W., Lennarz W. J. Metabolism of lipid-linked N-acetylglucosamine intermediates. J Biol Chem. 1977 May 25;252(10):3473–3479. [PubMed] [Google Scholar]
  4. Chen W. W., Lennarz W. J. Participation of a trisaccharide-lipid in glycosylation of oviduct membrane glycoproteins. J Biol Chem. 1976 Dec 25;251(24):7802–7809. [PubMed] [Google Scholar]
  5. Delmer D. P., Kulow C., Ericson M. C. Glycoprotein Synthesis in Plants: II. Structure of the Mannolipid Intermediate. Plant Physiol. 1978 Jan;61(1):25–29. doi: 10.1104/pp.61.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elbein A. D. Biosynthesis of a cell wall glucomannan in mung bean seedlings. J Biol Chem. 1969 Mar 25;244(6):1608–1616. [PubMed] [Google Scholar]
  7. Ericson M. C., Delmer D. P. Glycoprotein synthesis in plants: I. Role of lipid intermediates. Plant Physiol. 1977 Mar;59(3):341–347. doi: 10.1104/pp.59.3.341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ericson M. C., Gafford J. T., Elbein A. D. Tunicamycin inhibits GlcNAc-lipid formation in plants. J Biol Chem. 1977 Nov 10;252(21):7431–7433. [PubMed] [Google Scholar]
  9. Forsee W. T., Elbein A. D. Biosynthesis of mannosyl- and glucosyl-phosphoryl-polyprenols in cotton fibers. J Biol Chem. 1973 Apr 25;248(8):2858–2867. [PubMed] [Google Scholar]
  10. Forsee W. T., Elbein A. D. Glycoprotein biosynthesis in plants. Demonstration of lipid-linked oligosaccharides of mannose and N-acetylglucosamine. J Biol Chem. 1975 Dec 25;250(24):9283–9293. [PubMed] [Google Scholar]
  11. Heifetz A., Elbein A. D. Solubilization and properties of mannose and N-acetylglucosamine transferases involved in formation of polyprenyl-sugar intermediates. J Biol Chem. 1977 May 10;252(9):3057–3063. [PubMed] [Google Scholar]
  12. Lehle L., Fartaczek F., Tanner W., Kauss H. Formation of polyprenol-linked mono- and oligosaccharides in Phaseolus aureus. Arch Biochem Biophys. 1976 Aug;175(2):419–426. doi: 10.1016/0003-9861(76)90529-4. [DOI] [PubMed] [Google Scholar]
  13. Lennarz W. J. Lipid linked sugars in glycoprotein synthesis. Science. 1975 Jun 6;188(4192):986–991. doi: 10.1126/science.167438. [DOI] [PubMed] [Google Scholar]
  14. Lezica R. P., Brett C. T., Martinez P. R., Dankert M. A. A glucose acceptor in plants with the properties of an alpha-saturated polyprenyl-monophosphate. Biochem Biophys Res Commun. 1975 Oct 6;66(3):980–987. doi: 10.1016/0006-291x(75)90736-6. [DOI] [PubMed] [Google Scholar]
  15. Parodi A. J. Synthesis of glycosyl-dolichol derivatives in bakers' yeast and their role in protein glycosylation. Eur J Biochem. 1977 May 2;75(1):171–180. doi: 10.1111/j.1432-1033.1977.tb11514.x. [DOI] [PubMed] [Google Scholar]
  16. Smith M. M., Axelos M., Péaud-Lenoël C. Biosynthesis of mannan and mannolipids from GDP-Man by membrane fractions of sycamore cell cultures. Biochimie. 1976;58(10):1195–1211. doi: 10.1016/s0300-9084(76)80119-8. [DOI] [PubMed] [Google Scholar]
  17. Waechter C. J., Lennarz W. J. The role of polyprenol-linked sugars in glycoprotein synthesis. Annu Rev Biochem. 1976;45:95–112. doi: 10.1146/annurev.bi.45.070176.000523. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES