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. 1978 Mar;61(3):318–322. doi: 10.1104/pp.61.3.318

Distribution of Incorporated, Synthetic Cytokinins in Ribosomal RNA Preparations from Tobacco Callus 1

Norimoto Murai 1, Donald J Armstrong 1,2, Barbara J Taller 1, Folke Skoog 1
PMCID: PMC1091858  PMID: 16660283

Abstract

The distribution of incorporated synthetic cytokinins (N6-[8-14C]benzyladenine ([8-14C]bzl6Ade) and N6[8-14C]furfuryladenine ([8-14C]fr6Ade) in ribosomal RNA prepared from tobacco callus (Nicotiana tabacum L. var. Wis. No. 38) grown in the presence of one of these for 25 or 26 days has been studied. The rRNA of tissue supplied with [8-14C]bzl6Ade or [8-14C]fr6Ade was fractionated by methylated albumin-Kieselguhr column chromatography and preparative gel electrophoresis, respectively. In each case about 80% of the incorporated cytokinin was recovered as the ribonucleoside [8-14C]bzl6A or [8-14C]fr6A in the rRNA peak after the fractionations. [8-14C]fr6A was found associated with both the 18S and 25S rRNA components in quantities roughly proportional to their 260 nm absorbance. This pattern of apparently nonspecific association was not affected by prior denaturation of the RNA with formamide.

The distribution of [8-14C]fr6A moieties in mono- and oligonucleotides derived from combined treatment of [8-14C]fr6Ade-labeled rRNA with ribonuclease T1 and pancreatic ribonuclease A was measured by fractionating the digest on a DEAE-cellulose column in the presence of 7 m urea and determining the [8-14C]fr6A content in each fraction. The [8-14C]fr6A content in the oligonucleotides varied from 46 to 210 μmol/mol of adenosine (A). The mol ratio of [8-14C]fr6A to A was three to four times greater for oligonucleotides containing uridine or cytidine ([A]nUp or [A]nCp) than for those containing quanosine ([A]nGp).

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Selected References

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