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. 1978 Mar;61(3):339–341. doi: 10.1104/pp.61.3.339

Development and Properties of a Wax Ester Hydrolase in the Cotyledons of Jojoba Seedlings 1

Anthony H C Huang 1, Robert A Moreau 1,2, Kitty D F Liu 1
PMCID: PMC1091863  PMID: 16660288

Abstract

The activity of a wax ester hydrolase in the cotyledons of jojoba (Simmondsia chinensis) seedlings increased drastically during germination, parallel to the development of the gluconeogenic process. The enzyme at its peak of development was obtained in association with the wax body membrane, and its properties were studied. It had an optimal activity at alkaline pH (8.5-9). The apparent Km value for N-methylindoxylmyristate was 93 μM. It was stable at 40 C for 30 min but was inactivated at higher temperature. Various divalent cations and ethylenediaminetetraacetate had little effect on the activity. p-Chloromercuribenzoate was a strong inhibitor of the enzyme activity, and its effect was reversed by subsequent addition of dithiothreitol. It had a broad substrate specificity with highest activities on monoglycerides, wax esters, and the native substrate (jojoba wax).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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