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. 1978 Mar;61(3):465–468. doi: 10.1104/pp.61.3.465

Free Flow Electrophoresis of Chloroplasts

Jean-Paul Dubacq 1, Jean-Claude Kader 1
PMCID: PMC1091890  PMID: 16660315

Abstract

Highly purified intact chloroplasts were isolated from spinach (Spinacia oleracea L.) leaves by free flow electrophoresis. Morphological and biochemical studies showed that the fraction enriched in intact chloroplasts has a higher protein to chlorophyll ratio and a higher linolenic acid content than the broken organelles of the other fraction. The intact chloroplasts prepared by electrophoresis retained their capacity for CO2 fixation. Sodium dodecyl sulfate polyacrylamide gel electrophoresis demonstrated that this fraction was rich in stroma and lamellae proteins. Free flow electrophoresis, which separates organelles and molecules according to their surface charges, is a good technique for producing purified chloroplasts with complete physiological activities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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