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. 1978 Apr;61(4):703–705. doi: 10.1104/pp.61.4.703

Metabolic Pathway of α-Ketoglutarate in Citrus Leaves as Affected by Phosphorus Nutrition 1

Miriam Achituv 1, Avigdor Bar-Akiva 1
PMCID: PMC1091948  PMID: 16660367

Abstract

The uptake and metabolism of α-[5-14C]ketoglutarate by phosphorus-deficient and full nutrient (control) lemon (Citrus limon) leaves were studied over various time intervals. After 45 minutes in P-deficient leaves, the bulk of incorporated 14C appeared in organic acids and much less in amino acids, while in the control leaves, the 14C contents of organic and amino acids were equal. In P-deficient leaves, after longer incubation times the 14C content of organic acids and amino acids increased, while that of CO2 and residue fractions remained low. In full nutrient leaves the 14C content of amino acids and organic acids decreased after longer incubation time and increased in the insoluble residue and CO2. In full nutrient leaves the organic and amino acid metabolism were closely related and accompanied by protein synthesis and CO2 release, while in P-deficient leaves an accelerating accumulation of arginine and citric acid was linked together with inhibition of protein synthesis and CO2 liberation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bieleski R. L., Turner N. A. Separation and estimation of amino acids in crude plant extracts by thin-layer electrophoresis and chromatography. Anal Biochem. 1966 Nov;17(2):278–293. doi: 10.1016/0003-2697(66)90206-5. [DOI] [PubMed] [Google Scholar]
  2. Chou K. H., Splittstoesser W. E. Glutamate dehydrogenase from pumpkin cotyledons: characterization and isoenzymes. Plant Physiol. 1972 Apr;49(4):550–554. doi: 10.1104/pp.49.4.550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dougall D. K., Fulton M. M. Biosynthesis of protein amino acids in plant tissue culture. 3. Studies on the biosynthesis of arginine. Plant Physiol. 1967 Mar;42(3):387–390. doi: 10.1104/pp.42.3.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GLEITER M. E., PARKER H. E. The effect of phosphorus deficiency on the free amino acids of alfalfa. Arch Biochem Biophys. 1957 Oct;71(2):430–436. doi: 10.1016/0003-9861(57)90054-1. [DOI] [PubMed] [Google Scholar]
  5. Hautala E., Weaver M. L. Separation and quantitative determination of lactic, pyruvic, fumaric, succinic, malic, and citric acids by gas chromatography. Anal Biochem. 1969 Jul;30(1):32–39. doi: 10.1016/0003-2697(69)90370-4. [DOI] [PubMed] [Google Scholar]
  6. Morris C. J., Thompson J. F., Johnson C. M. Metabolism of Glutamic Acid and N-Acetylglutamic Acid in Leaf Discs and Cell-free Extracts of Higher Plants. Plant Physiol. 1969 Jul;44(7):1023–1026. doi: 10.1104/pp.44.7.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Thomas W. H., Krauss R. W. Nitrogen Metabolism in Scenedesmus as Affected by Environmental Changes. Plant Physiol. 1955 Mar;30(2):113–122. doi: 10.1104/pp.30.2.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Titus J. S., Splittstoesser W. E., Spencer P. Metabolism of alpha-Ketoglutarate by Roots of Woody Plants. Plant Physiol. 1968 Apr;43(4):619–621. doi: 10.1104/pp.43.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wiskich J. T. Phosphate-dependent Substrate Transport into Mitochondria: Oxidative Studies. Plant Physiol. 1975 Jul;56(1):121–125. doi: 10.1104/pp.56.1.121. [DOI] [PMC free article] [PubMed] [Google Scholar]

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