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. 1978 May;61(5):714–717. doi: 10.1104/pp.61.5.714

Factors Involved in in Vitro Stabilization of Nitrate Reductase from Cotton (Gossypium hirsutum L.) Cotyledons 1

Charles R Tischler 1,2, Albert C Purvis 1, Wayne R Jordan 1,3
PMCID: PMC1091963  PMID: 16660371

Abstract

Experiments were conducted to determine if pretreatment of cotton (Gossypium hirsutum L.) plants resulted in differential in vitro stabilities of nitrate reductase (NR) activity. Although NR activity declines markedly during the second half of the daily light period, in vitro NR stability is not modified by time of harvest. Phenylmethylsulfonylfluoride, iodoacetamide, and N-ethylmaleimide do not influence in vitro NR stability, suggesting that serine or sulfhydryl proteases are not responsible for in vitro lability of NR from cotton cotyledons.

Imposition of water stress or artificial extension of the dark period lead to significant reductions in NR activity, but do not change in vitro NR stability.

Dilution of a crude extract leads to increasing lability of NR; hence the marked instability of NR cannot be attributed to an inactivator which follows simple enzyme kinetics. Since in vitro NR activity is much more stable in presence of both NADH and NO3−, substrate availability must be considered as a possible factor influencing in vivo NR stability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beevers L., Schrader L. E., Flesher D., Hageman R. H. The Role of Light and Nitrate in the Induction of Nitrate Reductase in Radish Cotyledons and Maize Seedlings. Plant Physiol. 1965 Jul;40(4):691–698. doi: 10.1104/pp.40.4.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ferrari T. E., Yoder O. C., Filner P. Anaerobic nitrite production by plant cells and tissues: evidence for two nitrate pools. Plant Physiol. 1973 Mar;51(3):423–431. doi: 10.1104/pp.51.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hsiao T. C. Rapid Changes in Levels of Polyribosomes in Zea mays in Response to Water Stress. Plant Physiol. 1970 Aug;46(2):281–285. doi: 10.1104/pp.46.2.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Oaks A., Wallace W., Stevens D. Synthesis and turnover of nitrate reductase in corn roots. Plant Physiol. 1972 Dec;50(6):649–654. doi: 10.1104/pp.50.6.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Peterson L. W., Huffaker R. C. Loss of Ribulose 1,5-Diphosphate Carboxylase and Increase in Proteolytic Activity during Senescence of Detached Primary Barley Leaves. Plant Physiol. 1975 Jun;55(6):1009–1015. doi: 10.1104/pp.55.6.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Radin J. W. In vivo assay of nitrate reductase in cotton leaf discs: effect of oxygen and ammonium. Plant Physiol. 1973 Feb;51(2):332–336. doi: 10.1104/pp.51.2.332. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Scholander P. F., Bradstreet E. D., Hemmingsen E. A., Hammel H. T. Sap Pressure in Vascular Plants: Negative hydrostatic pressure can be measured in plants. Science. 1965 Apr 16;148(3668):339–346. doi: 10.1126/science.148.3668.339. [DOI] [PubMed] [Google Scholar]
  8. Scholl R. L., Harper J. E., Hageman R. H. Improvements of the nitrite color development in assays of nitrate reductase by phenazine methosulfate and zinc acetate. Plant Physiol. 1974 Jun;53(6):825–828. doi: 10.1104/pp.53.6.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Schrader L. E., Cataldo D. A., Peterson D. M. Use of protein in extraction and stabilization of nitrate reductase. Plant Physiol. 1974 May;53(5):688–690. doi: 10.1104/pp.53.5.688. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schrader L. E., Ritenour G. L., Eilrich G. L., Hageman R. H. Some characteristics of nitrate reductase from higher plants. Plant Physiol. 1968 Jun;43(6):930–940. doi: 10.1104/pp.43.6.930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shaner D. L., Boyer J. S. Nitrate Reductase Activity in Maize (Zea mays L.) Leaves: I. Regulation by Nitrate Flux. Plant Physiol. 1976 Oct;58(4):499–504. doi: 10.1104/pp.58.4.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith F. W., Thompson J. F. Regulation of nitrate reductase in excised barley roots. Plant Physiol. 1971 Aug;48(2):219–223. doi: 10.1104/pp.48.2.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Travis R. L., Jordan W. R., Huffaker R. C. Evidence for an Inactivating System of Nitrate Reductase in Hordeum vulgare L. during Darkness That Requires Protein Synthesis. Plant Physiol. 1969 Aug;44(8):1150–1156. doi: 10.1104/pp.44.8.1150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wallace W. Purification and properties of a nitrate reductase-inactivating enzyme. Biochim Biophys Acta. 1974 Mar 21;341(1):265–276. doi: 10.1016/0005-2744(74)90087-4. [DOI] [PubMed] [Google Scholar]
  15. Zielke H. R., Filner P. Synthesis and turnover of nitrate reductase induced by nitrate in cultured tobacco cells. J Biol Chem. 1971 Mar 25;246(6):1772–1779. [PubMed] [Google Scholar]

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