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. 1978 Jun;61(6):904–908. doi: 10.1104/pp.61.6.904

Metabolism of myo-Inositol by Germinating Lilium longiflorum Pollen 1

Alice E Manthey 1,2, David B Dickinson 1
PMCID: PMC1092009  PMID: 16660423

Abstract

Lilium Iongiflorum pollen tubes absorbed myo-[2-3H]inositol produced labeled metabolites which were separated into acid-soluble and -insoluble fractions. The soluble fraction contained labeled myo-inositol, d-glucuronic acid, myo-inositol 1-phosphate, and at least three other unidentified compounds. The acid-insoluble fraction contained considerable chloroformsoluble radioactivity and a labeled residue. Labeled myo-inositol was also absorbed by germinating pollen prior to the time of pollen tube initiation; however, there was a marked reduction in amounts of myo-inositol 1-phosphate and glucuronic acid produced by this pollen in comparison with growing pollen tubes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Chen M., Loewus F. A. myo-Inositol Metabolism in Lilium longiflorum Pollen: Uptake and Incorporation of myo-Inositol-2-H. Plant Physiol. 1977 Apr;59(4):653–657. doi: 10.1104/pp.59.4.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dickinson D. B., Hyman D., Gonzales J. W. Isolation of uridine 5'-pyrophosphate glucuronic Acid pyrophosphorylase and its assay using p-pyrophosphate. Plant Physiol. 1977 Jun;59(6):1082–1084. doi: 10.1104/pp.59.6.1082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dietz M., Albersheim P. The enzymic phosphorylation of myo-inositol. Biochem Biophys Res Commun. 1965 May 18;19(5):598–603. doi: 10.1016/0006-291x(65)90381-5. [DOI] [PubMed] [Google Scholar]
  5. English P. D., Dietz M., Albersheim P. Myoinositol kinase: partial purification and identification of product. Science. 1966 Jan 14;151(3707):198–199. doi: 10.1126/science.151.3707.198. [DOI] [PubMed] [Google Scholar]
  6. FEINGOLD D. S., NEUFELD E. F., HASSID W. Z. Enzymic synthesis of uridine diphosphate glucuronic acid and uridine diphosphate galacturonic acid with extracts from Phaseolus aureus seedlings. Arch Biochem Biophys. 1958 Dec;78(2):401–406. doi: 10.1016/0003-9861(58)90365-5. [DOI] [PubMed] [Google Scholar]
  7. Ikuma H., Tetley R. M. Possible Interference by an Acid-stable Enzyme during the Extraction of Nucleoside Di- and Triphosphates from Higher Plant Tissues. Plant Physiol. 1976 Sep;58(3):320–323. doi: 10.1104/pp.58.3.320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kindl H. Biosynthesis of epimers of myo-inositol, cyclohexanepentols, cyclohexenetetrols, and c-methylinositols. Ann N Y Acad Sci. 1969 Oct 17;165(2):615–623. [PubMed] [Google Scholar]
  9. Koller E., Koller F., Hoffmann-Ostenhof O. Myo-inositol oxygenase from oat seedlings. Mol Cell Biochem. 1976 Jan 31;10(1):33–39. doi: 10.1007/BF01731679. [DOI] [PubMed] [Google Scholar]
  10. Leibowitz M. D., Dickinson D. B., Loewus F. A., Loewus M. Partial purification and study of pollen glucuronokinase. Arch Biochem Biophys. 1977 Mar;179(2):559–564. doi: 10.1016/0003-9861(77)90144-8. [DOI] [PubMed] [Google Scholar]
  11. Loewus F. Metabolism of inositol in higher plants. Ann N Y Acad Sci. 1969 Oct 17;165(2):577–598. [PubMed] [Google Scholar]

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