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. 1978 Jun;61(6):957–960. doi: 10.1104/pp.61.6.957

Effects of Inhibitors of Catalase on Photosynthesis and on Catalase Activity in Unwashed Preparations of Intact Chloroplasts

John F Allen 1, F R Whatley 1
PMCID: PMC1092020  PMID: 16660434

Abstract

The catalase activity of unwashed preparations containing intact spinach (Spinacia oleracea L.) chloroplasts is inhibited both by cyanide and by azide at concentrations which also cause inhibition of photosynthetic CO2- dependent O2 evolution.

Aminotriazole can also be used to inhibit this contaminant catalase, and in this case inhibition of catalase can be achieved at aminotriazole concentrations which have little effect on the rate of photosynthetic CO2 fixation. Aminotriazole may be used as a specific inhibitor of catalase in order to demonstrate inhibition of photosynthesis by added H2O2.

It is therefore concluded that inhibition of photosynthesis by cyanide and azide does not necessarily result from inhibition of catalase in the chloroplast preparation, and that intact chloroplasts do not produce inhibitory concentrations of H2O2 under the best experimental conditions for CO2 fixation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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