Abstract
Cytidine deoxyriboside (Cdr), uridine deoxyriboside (Udr), and guanosine deoxyriboside (Gdr), induce quantitative bleaching of the fronds of Lemna minor (duckweed) during growth in continuous light on photoheterotrophic medium. Cdr-induced bleaching is not accompanied by a reduction in frond multiplication rate, but Udr- and Gdr-induced bleaching is. Bleaching by Cdr is fully prevented by thymidine (Tdr), cytidine (Cr), or uridine (Ur), but not by orotic acid (OA) which itself inhibits growth. Bleaching by Udr is not antagonized by Tdr, Cdr, Cr, Ur, or OA. The ability of Cdr to induce phenocopies of chlorophyll-deficient mutants in the absence of effect on growth rate is interpreted as indicating a functional compartmentation of pyrimidine metabolism between chloroplast and whole cell. On the assumption that Cdr induces bleaching by regulating the biosynthesis of deoxynucleoside triphosphates, and in analogy with the antagonism of fluorodeoxyuridine effects on growth by Tdr, Cr, or Ur, the suggestion is made that deoxycytidine is converted to thymidylate by a step other than that utilizing thymidylate synthetase.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dale B. A., Greenberg G. R. Effect of the folic acid analogue, trimethoprim, on growth, macromolecular synthesis, and incorporation of exogenous thymine in Escherichia coli. J Bacteriol. 1972 Jun;110(3):905–916. doi: 10.1128/jb.110.3.905-916.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deng Q. I., Ives D. H. Modes of nucleoside phosphorylation in plants: studies on the apparent thymidine kinase and true uridine kinase of seedlings. Biochim Biophys Acta. 1972 Aug 25;277(2):235–244. [PubMed] [Google Scholar]
- Friedkin M. Thymidylate synthetase. Adv Enzymol Relat Areas Mol Biol. 1973;38:235–292. doi: 10.1002/9780470122839.ch5. [DOI] [PubMed] [Google Scholar]
- Frisch D. M., Charles M. A. Deamination of 4-aminopyrimidine nucleosides by extracts of rye grass (Lolium perenne). Plant Physiol. 1966 Mar;41(3):475–478. doi: 10.1104/pp.41.3.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goody H. E., Ellem K. A. Nutritional effects on precursor uptake and compartmentalization of intracellular pools in relation to RNA synthesis. Biochim Biophys Acta. 1975 Feb 24;383(1):30–39. doi: 10.1016/0005-2787(75)90243-9. [DOI] [PubMed] [Google Scholar]
- Heidelberger C. Fluorinated pyrimidines. Prog Nucleic Acid Res Mol Biol. 1965;4:1–50. doi: 10.1016/s0079-6603(08)60783-7. [DOI] [PubMed] [Google Scholar]
- Kuebbing D., Werner R. A model for compartmentation of de novo and salvage thymidine nucleotide pools in mammalian cells. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3333–3336. doi: 10.1073/pnas.72.9.3333. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Donovan G. A., Neuhard J. Pyrimidine metabolism in microorganisms. Bacteriol Rev. 1970 Sep;34(3):278–343. doi: 10.1128/br.34.3.278-343.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ross C., Murray M. G. Development of Pyrimidine-metabolizing Enzymes in Cotyledons of Germinating Peas. Plant Physiol. 1971 Nov;48(5):626–630. doi: 10.1104/pp.48.5.626. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rédei G. P. Arabidopsis as a genetic tool. Annu Rev Genet. 1975;9:111–127. doi: 10.1146/annurev.ge.09.120175.000551. [DOI] [PubMed] [Google Scholar]
- Van Overbeek J., Loeffler J. E., Mason M. I. Dormin (Abscisin II), inhibitor of plant DNA synthesis? Science. 1967 Jun 16;156(3781):1497–1499. doi: 10.1126/science.156.3781.1497. [DOI] [PubMed] [Google Scholar]
- Wiegers U., Kramer G., Klapproth K., Hilz H. Separate pyrimidine-nucleotide pools for messenger-RNA and ribosomal-RNA synthesis in HeLa S3 cells. Eur J Biochem. 1976 May 1;64(2):535–540. doi: 10.1111/j.1432-1033.1976.tb10333.x. [DOI] [PubMed] [Google Scholar]