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. 1978 Jul;62(1):22–25. doi: 10.1104/pp.62.1.22

Oxidation of Proline by Plant Mitochondria 1

Samuel F Boggess 1,2, David E Koeppe 1,2, Cecil R Stewart 1,2
PMCID: PMC1092047  PMID: 16660461

Abstract

Mitochondria isolated from etiolated shoots of corn (Zea mays), wheat (Triticum aestivum), barley (Hordeum vulgare), soybean (Glycine max L. Merr.), and mung bean (Phaseolus aureus) exhibited a proline-dependent O2 uptake subject to respiratory control. ADP/O ratios with proline as substrate were intermediate between ratios obtained with exogenous NADH and malate + pyruvate as substrates. Isotope studies showed proline metabolism to be dependent on O2, but not NAD. The major ninhydrin-positive product formed via Δ1-pyrroline-5-carboxylic acid was glutamate. Mitochondria were capable of further metabolism of glutamate, as radioactive CO2, organic acids, and aspartate were recovered after [14C]proline feeding experiments. These results demonstrate the mitochondrial association and O2 dependence of plant proline metabolism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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