Abstract
Mitochondria isolated from etiolated shoots of corn (Zea mays), wheat (Triticum aestivum), barley (Hordeum vulgare), soybean (Glycine max L. Merr.), and mung bean (Phaseolus aureus) exhibited a proline-dependent O2 uptake subject to respiratory control. ADP/O ratios with proline as substrate were intermediate between ratios obtained with exogenous NADH and malate + pyruvate as substrates. Isotope studies showed proline metabolism to be dependent on O2, but not NAD. The major ninhydrin-positive product formed via Δ1-pyrroline-5-carboxylic acid was glutamate. Mitochondria were capable of further metabolism of glutamate, as radioactive CO2, organic acids, and aspartate were recovered after [14C]proline feeding experiments. These results demonstrate the mitochondrial association and O2 dependence of plant proline metabolism.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barnett N. M., Naylor A. W. Amino Acid and protein metabolism in bermuda grass during water stress. Plant Physiol. 1966 Sep;41(7):1222–1230. doi: 10.1104/pp.41.7.1222. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boggess S. F., Stewart C. R. Effect of water stress on proline synthesis from radioactive precursors. Plant Physiol. 1976 Sep;58(3):398–401. doi: 10.1104/pp.58.3.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FRANK L., RANHAND B. PROLINE METABOLISM IN ESCHERICHIA COLI. 3. THE PROLINE CATABOLIC PATHWAY. Arch Biochem Biophys. 1964 Aug;107:325–331. doi: 10.1016/0003-9861(64)90338-8. [DOI] [PubMed] [Google Scholar]
- Gamper H., Moses V. Enzyme organization in the proline biosynthetic pathway of Escherichia coli. Biochim Biophys Acta. 1974 Jun 20;354(1):75–87. doi: 10.1016/0304-4165(74)90055-5. [DOI] [PubMed] [Google Scholar]
- JOHNSON A. B., STRECKER H. J. The interconversion of glutamic acid and proline. IV. The oxidation of proline by rat liver mitochondria. J Biol Chem. 1962 Jun;237:1876–1882. [PubMed] [Google Scholar]
- LING C. M., HEDRICK L. R. PROLINE OXIDASES IN HANSENULA SUBPELLICULOSA. J Bacteriol. 1964 Jun;87:1462–1470. doi: 10.1128/jb.87.6.1462-1470.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- McNamer A. D., Stewart C. R. Nicotinamide Adenine Dinucleotide-dependent Proline Dehydrogenase in Chlorella. Plant Physiol. 1974 Mar;53(3):440–444. doi: 10.1104/pp.53.3.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller R. J., Dumford S. W., Koeppe D. E., Hanson J. B. Divalent cation stimulation of substrate oxidation by corn mitochondria. Plant Physiol. 1970 Jun;45(6):649–653. doi: 10.1104/pp.45.6.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitra R., Gross R. D., Varner J. E. An intact assay for enzymes that labilize C--H bonds. Anal Biochem. 1975 Mar;64(1):102–109. doi: 10.1016/0003-2697(75)90410-8. [DOI] [PubMed] [Google Scholar]
- Morris C. J., Thompson J. F., Johnson C. M. Metabolism of Glutamic Acid and N-Acetylglutamic Acid in Leaf Discs and Cell-free Extracts of Higher Plants. Plant Physiol. 1969 Jul;44(7):1023–1026. doi: 10.1104/pp.44.7.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PEISACH J., STRECKER H. J. The interconversion of glutamic acid and proline. V. The reduction of delta 1-pyrroline-5-carboxylic acid to proline. J Biol Chem. 1962 Jul;237:2255–2260. [PubMed] [Google Scholar]
- Phang J. M., Downing S. J., Valle D. L., Kowaloff E. M. A radioisotopic assay for proline oxidase activity. J Lab Clin Med. 1975 Feb;85(2):312–317. [PubMed] [Google Scholar]
- Stewart C. R. Inhibition of proline oxidation by water stress. Plant Physiol. 1977 May;59(5):930–932. doi: 10.1104/pp.59.5.930. [DOI] [PMC free article] [PubMed] [Google Scholar]