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. 1978 Jul;62(1):40–43. doi: 10.1104/pp.62.1.40

Isolation of Intact Plastids from Protoplasts from Castor Bean Endosperm 1

Mikio Nishimura 1,2, Harry Beevers 1
PMCID: PMC1092051  PMID: 16660465

Abstract

Protoplasts were prepared from castor bean (Ricinus communis) endosperm by treatment with a mixture of the commercial enzymes Macerozyme R-10 and Cellulose “Onozuka” R-10. The protoplasts were gently ruptured by forcing the suspension through a hypodermic needle and the homogenate centrifuged on a linear sucrose gradient. From such a homogenate the mitochondria are recovered at their typical isopycnic density of 1.18 g/ml, but the glyoxysomes are retained, with other membranes, at a density of 1.13. The plastids reach their typical density of 1.22 on the gradient and are thus clearly separated from other organelles. Moreover, since essentially all of the ribulose bisphosphate carboxylase activity on the gradient is present in this fraction it can be concluded that the plastids are intact and have been recovered in high yield.

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41FIG. 1
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42FIG. 2
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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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