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. 1978 Jul;62(1):44–48. doi: 10.1104/pp.62.1.44

Hydrolases in Vacuoles from Castor Bean Endosperm 1

Mikio Nishimura 1,2, Harry Beevers 1
PMCID: PMC1092052  PMID: 16660466

Abstract

Vacuoles were prepared from endosperm tissue of 4-day-old castor bean seedlings (Ricinus communis var. Hale) and purified on a stepped sucrose gradient. It was shown by assays of marker enzymes that there was only trace contamination of the final preparation by other organelles (mitochondria, glyoxysomes, nuclei, spherosomes, and plastids) and by cytoplasmic components. Hydrolytic enzymes (acid protease, carboxypeptidase, phosphodiesterase, RNAase, phytase and β-glucosidase) were present in the isolated vacuoles in amounts indicating a primarily vacuolar localization in vivo. The vacuoles also contained storage protein and high concentrations of sucrose. The over-all results indicate that the vacuoles from castor bean endosperm are the site of hydrolysis of the constituents of the protein bodies and are a temporary storage compartment for the sucrose produced from fat and protein reserves.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asahi T., Nishimura M. Regulatory function of malate dehydrogenase isoenzymes in the cotyledons of mung bean. J Biochem. 1973 Feb;73(2):217–225. [PubMed] [Google Scholar]
  2. Butcher H. C., Wagner G. J., Siegelman H. W. Localization of Acid hydrolases in protoplasts: examination of the proposed lysosomal function of the mature vacuole. Plant Physiol. 1977 Jun;59(6):1098–1103. doi: 10.1104/pp.59.6.1098. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  4. Leigh R. A., Branton D. Isolation of Vacuoles from Root Storage Tissue of Beta vulgaris L. Plant Physiol. 1976 Nov;58(5):656–662. doi: 10.1104/pp.58.5.656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lord J. M., Kagawa T., Moore T. S., Beevers H. Endoplasmic reticulum as the site of lecithin formation in castor bean endosperm. J Cell Biol. 1973 Jun;57(3):659–667. doi: 10.1083/jcb.57.3.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Muto S., Beevers H. Lipase Activities in Castor Bean Endosperm during Germination. Plant Physiol. 1974 Jul;54(1):23–28. doi: 10.1104/pp.54.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nishimura M., Takabe T., Sugiyama T., Akazawa T. Structure and function of chloroplast proteins. XIX. Dissociation of spinach leaf ribulose-1,5-diphosphate carboxylase by p-mercuribenzoate. J Biochem. 1973 Nov;74(5):945–954. [PubMed] [Google Scholar]
  8. Osmond C. B., Akazawa T., Beevers H. Localization and properties of ribulose diphosphate carboxylase from castor bean endosperm. Plant Physiol. 1975 Feb;55(2):226–230. doi: 10.1104/pp.55.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RACKER E. Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids. Biochim Biophys Acta. 1950 Jan;4(1-3):211–214. doi: 10.1016/0006-3002(50)90026-6. [DOI] [PubMed] [Google Scholar]
  10. ROSEN H. A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys. 1957 Mar;67(1):10–15. doi: 10.1016/0003-9861(57)90241-2. [DOI] [PubMed] [Google Scholar]
  11. Tully R. E., Beevers H. Protein bodies of castor bean endosperm: isolation, fractionation, and the characterization of protein components. Plant Physiol. 1976 Dec;58(6):710–716. doi: 10.1104/pp.58.6.710. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Van Handel E. Direct microdetermination of sucrose. Anal Biochem. 1968 Feb;22(2):280–283. doi: 10.1016/0003-2697(68)90317-5. [DOI] [PubMed] [Google Scholar]
  13. Walker-Simmons M., Ryan C. A. Immunological Identification of Proteinase Inhibitors I and II in Isolated Tomato Leaf Vacuoles. Plant Physiol. 1977 Jul;60(1):61–63. doi: 10.1104/pp.60.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. van Rijn H. J., Linnemans W. A., Boer P. Localization of acid phosphatase in protoplasts from Saccharomyces cerevisiae. J Bacteriol. 1975 Sep;123(3):1144–1149. doi: 10.1128/jb.123.3.1144-1149.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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