Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1978 Sep;62(3):348–353. doi: 10.1104/pp.62.3.348

Identification of an Acyl Donor in Steryl Ester Biosynthesis by Enzyme Preparations from Spinach Leaves

Raymond E Garcia 1,1,2, J Brian Mudd 1
PMCID: PMC1092124  PMID: 16660515

Abstract

A pathway for steryl ester biosynthesis in acetone powder preparations from spinach (Spinacia oleracea L.) leaves has been elucidated; free sterol and 1,2-diglyceride were the substrates. Although animals synthesize cholesteryl esters by three distinct biosynthetic pathways, none of these pathways utilizes 1,2-diglyceride as an acyl donor. Phosphatidylcholine, phosphatidic acid, triglyceride, 1,3-diglyceride, 1-monoglyceride, free fatty acid, and fatty acyl-CoA were not acyl donors for spinach leaf steryl ester biosynthesis in our assay system. The unstable 2 isomer of monoglyceride was not tested. It is possible that 1,2-diglyceride and 2-monoglyceride were both acyl donors for spinach leaf steryl ester biosynthesis. Acyl-labeled phosphatidylcholine and acyl-labeled phosphatidylethanolamine were rapidly degraded by acetone powder preparations to 1,2-diglyceride via phosphatidic acid. The 1,2-diglycerides were slowly metabolized to monoglycerides, triglycerides, free fatty acids, and steryl esters. The monoglycerides were rapidly degraded to free fatty acids and glycerol.

Full text

PDF
348

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. CHENIAE G. M. PHOSPHATIDIC ACID AND GLYCERIDE SYNTHESIS BY PARTICLES FROM SPINACH LEAVES. Plant Physiol. 1965 Mar;40:235–243. doi: 10.1104/pp.40.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GOODMAN D. S., DEYKIN D., SHIRATORI T. THE FORMATION OF CHOLESTEROL ESTERS WITH RAT LIVER ENZYMES. J Biol Chem. 1964 May;239:1335–1345. [PubMed] [Google Scholar]
  4. Hyun J., Kothari H., Herm E., Mortenson J., Treadwell C. R., Vahouny G. V. Purification and properties of pancreatic juice cholesterol esterase. J Biol Chem. 1969 Apr 10;244(7):1937–1945. [PubMed] [Google Scholar]
  5. KATES M. Lecithinase systems in sugar beet, spinach, cabbage, and carrot. Can J Biochem Physiol. 1954 Sep;32(5):571–583. [PubMed] [Google Scholar]
  6. Knoche H., Esders T. W., Koths K., Bloch K. Palmityl coenzyme A inhibition of fatty acid synthesis. Relief by bovine serum albumin and mycobacterial polysaccharides. J Biol Chem. 1973 Apr 10;248(7):2317–2322. [PubMed] [Google Scholar]
  7. Kolattukudy P. E. Mechanisms of synthesis of waxy esters in broccoli (Brassica oleracea). Biochemistry. 1967 Sep;6(9):2705–2717. doi: 10.1021/bi00861a010. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Moore T. S., Lord J. M., Kagawa T., Beevers H. Enzymes of phospholipid metabolism in the endoplasmic reticulum of castor bean endosperm. Plant Physiol. 1973 Jul;52(1):50–53. doi: 10.1104/pp.52.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Quarles R. H., Dawson R. M. The distribution of phospholipase D in developing and mature plants. Biochem J. 1969 May;112(5):787–794. doi: 10.1042/bj1120787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Taketa K., Pogell B. M. The effect of palmityl coenzyme A on glucose 6-phosphate dehydrogenase and other enzymes. J Biol Chem. 1966 Feb 10;241(3):720–726. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES