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. 1978 Oct;62(4):522–525. doi: 10.1104/pp.62.4.522

Modification of Disease Resistance of Tobacco Callus Tissues by Cytokinins 1

Geraldine T Haberlach 1,2, Allen D Budde 1, Luis Sequeira 1, John P Helgeson 1,2
PMCID: PMC1092163  PMID: 16660551

Abstract

The effects of differing cytokinin and auxin concentrations on resistance of tobacco (Nicotiana tabacum L.) tissue cultures to race 0 of Phytophthora parasitica var. nicotianae were examined. With 1 micromolar kinetin and either 11.5 micromolar indoleacetic acid or 1 micromolar 2,4-dichlorophen-oxyacetic acid, tissues from resistant cultivars exhibited a “hypersensitive” reaction to zoospores of the fungus and subsequently were colonized only slightly. With susceptible cultivars or with tissues from resistant cultivars supplied with higher cytokinin levels (e.g. 10 micromolar kinetin), this hypersensitive reaction did not occur and tissues were heavily colonized. Benzylaminopurine and kinetin were particularly effective in eliminating both the hypersensitive reaction and disease resistance. Zeatin and 6-(3-methyl-2-butenylamino)purine were less effective. Increases in indoleacetic acid levels reversed the effects of high cytokinin concentrations. The balance of phytohormones apparently controls the host response to the fungus; thus, in this system, resistance or susceptibility can be studied without changing either host or fungal genotype.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. HAMZI H. Q., SKOOG F. KINETIN-LIKE GROWTH-PROMOTING ACTIVITY OF 1-SUBSTITUTED ADENINES (1-BENZYL-6-AMINOPURINE AND 1-(GAMMA, GAMMA-DIMETHYLALLYL)-6-AMINOPURINE. Proc Natl Acad Sci U S A. 1964 Jan;51:76–83. doi: 10.1073/pnas.51.1.76. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Jackson A. O., Larkins B. A. Influence of Ionic Strength, pH, and Chelation of Divalent Metals on Isolation of Polyribosomes from Tobacco Leaves. Plant Physiol. 1976 Jan;57(1):5–10. doi: 10.1104/pp.57.1.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Tetley R. M., Thimann K. V. The Metabolism of Oat Leaves during Senescence: I. Respiration, Carbohydrate Metabolism, and the Action of Cytokinins. Plant Physiol. 1974 Sep;54(3):294–303. doi: 10.1104/pp.54.3.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Witham F. H. Effect of 2,4-dichlorophenoxyacetic Acid on the cytokinin requirement of soybean cotyledon and tobacco stem pith callus tissues. Plant Physiol. 1968 Sep;43(9):1455–1457. doi: 10.1104/pp.43.9.1455. [DOI] [PMC free article] [PubMed] [Google Scholar]

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