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. 1978 Oct;62(4):590–597. doi: 10.1104/pp.62.4.590

Apparent Catalase Synthesis in Sunflower Cotyledons during the Change in Microbody Function

A Mathematical Approach for the Quantitative Evaluation of Density-labeling Data 1

Thomas Betsche 1, Bernt Gerhardt 1
PMCID: PMC1092177  PMID: 16660565

Abstract

Density-labeling with 10 mm K15NO3/70% 2H2O has been used to investigate catalase synthesis in different developmental stages of sunflower (Helianthus annuus L.) cotyledons. A mathematical approach is introduced for the quantitative evaluation of the density-labeling data. The method allows, in the presence of preexisting enzyme activity, calculation of this synthesized activity (apparent enzyme synthesis) which results from the balance between actual enzyme synthesis and the degradation of newly synthesized enzyme at a given time. During greening of the cotyledons, when the catalase activity declines and the population of leaf peroxisomes is formed, the apparent catalase synthesis is lower than, or at best equal to, that occurring during a developmental stage when the leaf peroxisome population is established and catalase synthesis and degradation of total catalase are in equilibrium. This result suggests a formation, in fatty cotyledons, of the leaf peroxisomes by transformation of the glyoxysomes rather than by de novo synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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