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. 1978 Nov;62(5):761–765. doi: 10.1104/pp.62.5.761

Regulation of Ribosomal RNA Accumulation by Auxin in Artichoke Tissue 1

Dara L Melanson 1,2,2, John Ingle 1,2
PMCID: PMC1092216  PMID: 16660601

Abstract

Artichoke (Helianthus tuberosus L.) tuber tissue cultured in the presence of the auxin 2,4-dichlorophenoxyacetic acid accumulates ribosomal RNA at a rate of 0.135 micrograms per hour per explant whereas there is little accumulation in nontreated tissue. The addition of auxin enhanced the transcription of the 2.5 × 106 precursor 3.5-fold and increased the rate of processing 1.8-fold. The major effect of auxin, however, was a vast increase in the rate of processing of the 1.39 × 106 precursor to the 1.3 × 106 mature ribosomal RNA. The incorporation of label into the 0.7 × 106 mature ribosomal RNA of treated tissue was in 10-fold excess over the control after a 30-minute pulse and remained so throughout the remainder of the labeling period. This level, however, was not reached for the complementary 1.3 mature RNA until 3 hours of continuous labeling, decreasing from a initial value of 40-fold excess. A complication in the processing of ribosomal RNA is the apparent increase in the stability of the 0.7 × 106 mature RNA with auxin treatment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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