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. 1978 Nov;62(5):766–772. doi: 10.1104/pp.62.5.766

Membrane-associated Glycosyl Transferases in Cotyledons of Pisum sativum

Differential Effects of Magnesium and Manganese Ions 1

Jerry Nagahashi 1, Rose M Mense 1, Leonard Beevers 1
PMCID: PMC1092217  PMID: 16660602

Abstract

In crude particulate fractions isolated from pea (Pisum sativum) cotyledons, the transfer of radioactivity from GDP-[14C]mannose to glycolipid appears to be preferentially stimulated by Mn2+ while the transfer to lipid-free residue is enhanced by Mg2+. In contrast, the transfer of radioactivity from UDP-N-acetyl-[14C]glucosamine to glycolipid shows preferential stimulation by Mg2+ while the transfer to lipid-free residue prefers Mn2+. These results are accounted for by the differential stimulation by Mg2+ and Mn2+ of glycosyl transferases associated with subcellular membranes which were separated by isopycnic sucrose density centrifugation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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