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. 1978 Nov;62(5):838–840. doi: 10.1104/pp.62.5.838

Capping Structures at the 5′-Terminus of Polyadenylated Ribonucleic Acid in Avena Coleoptiles

Richard A Haugland 1,2,1, Morris G Cline 1,2
PMCID: PMC1092234  PMID: 16660619

Abstract

Evidence is presented for the occurrence of 5′-terminal capping structures in the polyadenylated RNA of oat (Avena sativa) coleoptiles. These structures are composed of an inverted terminal nucleoside containing the modified base 7-methylguanine which is joined 5′ to 5′ with a second (penultimate) nucleoside by means of three phosphate groups in two pyrophosphate linkages. The penultimate nucleoside is joined to the remainder of the RNA molecule by a conventional 3′,5′ phosphodiester bond. A significant difference between the cap structures of oat coleoptile RNA and those of previously described higher eucaryotic cellular mRNAs is the lack of ribose methylations in the penultimate nucleosides of the plant RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham G., Rhodes D. P., Banerjee A. K. The 5' terminal structure of the methylated mRNA synthesized in vitro by vesicular stomatitis virus. Cell. 1975 May;5(1):51–58. doi: 10.1016/0092-8674(75)90091-4. [DOI] [PubMed] [Google Scholar]
  2. Colonno R. J., Stone H. O. Newcastle disease virus mRNA lacks 2'-O-methylated nucleotides. Nature. 1976 Jun 17;261(5561):611–614. doi: 10.1038/261611a0. [DOI] [PubMed] [Google Scholar]
  3. Dasgupta R., Harada F., Kaesberg P. Blocked 5' termini in brome mosaic virus RNA. J Virol. 1976 Apr;18(1):260–267. doi: 10.1128/jvi.18.1.260-267.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dottin R. P., Weiner A. M., Lodish F. 5' terminal nucleotide sequences of the messenger RNA's of Dictyostelium discoideum. Cell. 1976 Jun;8(2):233–244. doi: 10.1016/0092-8674(76)90007-6. [DOI] [PubMed] [Google Scholar]
  5. Dubin D. T., Stollar V. Methylation of Sindbis virus "26S" messenger RNA. Biochem Biophys Res Commun. 1975 Oct 27;66(4):1373–1379. doi: 10.1016/0006-291x(75)90511-2. [DOI] [PubMed] [Google Scholar]
  6. Furuichi Y., Shatkin A. J., Stavnezer E., Bishop J. M. Blocked, methylated 5'-terminal sequence in avian sarcoma virus RNA. Nature. 1975 Oct 16;257(5527):618–620. doi: 10.1038/257618a0. [DOI] [PubMed] [Google Scholar]
  7. Kennedy T. D., Lane B. G. The probable 'capping' of wheat leaf messenger ribonucleates by 7-methylguanosine. Can J Biochem. 1975 Dec;53(12):1346–1348. doi: 10.1139/o75-184. [DOI] [PubMed] [Google Scholar]
  8. Moyer S. A., Abraham G., Adler R., Banerjee A. K. Methylated and blocked 5' termini in vesicular stomatitis virus in vivo mRNAs. Cell. 1975 May;5(1):59–67. doi: 10.1016/0092-8674(75)90092-6. [DOI] [PubMed] [Google Scholar]
  9. Muthukrishnan S., Both G. W., Furuichi Y., Shatkin A. J. 5'-Terminal 7-methylguanosine in eukaryotic mRNA is required for translation. Nature. 1975 May 1;255(5503):33–37. doi: 10.1038/255033a0. [DOI] [PubMed] [Google Scholar]
  10. Muthukrishnan S., Morgan M., Banerjee A. K., Shatkin A. J. Influence of 5'-terminal m7G and 2'--O-methylated residues on messenger ribonucleic acid binding to ribosomes. Biochemistry. 1976 Dec 28;15(26):5761–5768. doi: 10.1021/bi00671a012. [DOI] [PubMed] [Google Scholar]
  11. Perry R. P., Kelley D. E. Kinetics of formation of 5' terminal caps in mRNA. Cell. 1976 Jul;8(3):433–442. doi: 10.1016/0092-8674(76)90156-2. [DOI] [PubMed] [Google Scholar]
  12. Perry R. P., Kelley D. E. Methylated constituents of heterogeneous nuclear RNA: presence in blocked 5' terminal structures. Cell. 1975 Sep;6(1):13–19. doi: 10.1016/0092-8674(75)90068-9. [DOI] [PubMed] [Google Scholar]
  13. Pinck L. The 5'-end groups of alfalfa mosaic virus RNAs are m7G5'ppp5'Gp. FEBS Lett. 1975 Nov 1;59(1):24–28. doi: 10.1016/0014-5793(75)80332-2. [DOI] [PubMed] [Google Scholar]
  14. Rhodes D. P., Reddy D. V., MacLeod R., Black L. M., Banerjee A. K. In vitro synthesis of RNA containing 5'-terminal structure 7nG(5')ppp(5')Apm... by purified wound tumor virus. Virology. 1977 Feb;76(2):554–559. doi: 10.1016/0042-6822(77)90237-9. [DOI] [PubMed] [Google Scholar]
  15. Saini M. S., Lane B. G. Wheat embryo ribonucleates. VIII. The presence of 7-methylguanosine 'cap structures' in the RNA of imbibing wheat embryos. Can J Biochem. 1977 Aug;55(8):819–824. doi: 10.1139/o77-121. [DOI] [PubMed] [Google Scholar]
  16. Schibler U., Perry R. P. Characterization of the 5' termini of hn RNA in mouse L cells: implications for processing and cap formation. Cell. 1976 Sep;9(1):121–130. doi: 10.1016/0092-8674(76)90058-1. [DOI] [PubMed] [Google Scholar]
  17. Shatkin A. J. Capping of eucaryotic mRNAs. Cell. 1976 Dec;9(4 Pt 2):645–653. doi: 10.1016/0092-8674(76)90128-8. [DOI] [PubMed] [Google Scholar]
  18. Sripati C. E., Groner Y., Warner J. R. Methylated, blocked 5' termini of yeast mRNA. J Biol Chem. 1976 May 25;251(10):2898–2904. [PubMed] [Google Scholar]
  19. Zimmern D. The 5' end group of tobacco mosaic virus RNA is m7G5' ppp5' Gp. Nucleic Acids Res. 1975 Jul;2(7):1189–1201. doi: 10.1093/nar/2.7.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]

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