Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1978 Dec;62(6):841–845. doi: 10.1104/pp.62.6.841

Determination of Indole-3-acetic Acid in Douglas Fir Using a Deuterated Analog and Selected Ion Monitoring

Comparison of Microquantities in Seedling and Adult Tree 1

John L Caruso 1,2, Ronald G Smith 1,3, Lawrence M Smith 1,4, Tsai-Ying Cheng 1, G Doyle Daves Jr 1
PMCID: PMC1092238  PMID: 16660622

Abstract

Indole-3-acetic acid (IAA) content in shoot tips of Douglas fir (Pseudotsuga menziesii [Mirb.] Franco) trees and seedlings was determined by combined gas chromatography-mass spectrometry using a deuterated analog (d2-IAA) as an internal standard and the technique of selected ion monitoring. Ratios of the peak heights of the deuterated analog internal standard to endogenous IAA revealed a slightly higher content of IAA in seedlings compared with the shoot tips collected in June. The relatively high level of IAA (2.9 micrograms per gram fresh weight) in seedlings is discussed in relation to in vitro propagation of this species.

Full text

PDF
841

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bitancourt A. A. Reversible inactivation of aged solutions of indolyl-3-acetic Acid. Science. 1966 Dec 9;154(3754):1327–1329. doi: 10.1126/science.154.3754.1327. [DOI] [PubMed] [Google Scholar]
  2. Cheng T. Y., Voqui T. H. Regeneration of douglas fir plantlets through tissue culture. Science. 1977 Oct 21;198(4314):306–307. doi: 10.1126/science.198.4314.306. [DOI] [PubMed] [Google Scholar]
  3. Deyoe D. R., Zaerr J. B. Indole-3-acetic Acid in Douglas Fir: Analysis by Gas-Liquid Chromatography and Mass Spectrometry. Plant Physiol. 1976 Sep;58(3):299–303. doi: 10.1104/pp.58.3.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hoskins J. A., Pollitt R. J. Quantitative aspects of urinary indolo-3-acetic acid and 5-hydroxyindole-3-acetic acid excretion. J Chromatogr. 1975 Jun 18;109(2):436–438. doi: 10.1016/s0021-9673(01)91824-2. [DOI] [PubMed] [Google Scholar]
  5. Seeley S. D., Powell L. E. Gas chromatography and detection of microquantities of gibberellins and indoleacetic acid as their fluorinated derivatives. Anal Biochem. 1974 Mar;58(1):39–46. doi: 10.1016/0003-2697(74)90438-2. [DOI] [PubMed] [Google Scholar]
  6. Smith R. G., Daves G. D., Jr Gas chromatography mass spectrometry analysis of polyamines using deuterated analogs as internal standards. Biomed Mass Spectrom. 1977 Jun;4(3):146–151. doi: 10.1002/bms.1200040305. [DOI] [PubMed] [Google Scholar]
  7. Sweetser P. B., Swartzfager D. G. Indole-3-acetic Acid Levels of Plant Tissue as Determined by a New High Performance Liquid Chromatographic Method. Plant Physiol. 1978 Feb;61(2):254–258. doi: 10.1104/pp.61.2.254. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES