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. 1978 Dec;62(6):907–911. doi: 10.1104/pp.62.6.907

Within-day Changes in the Polyribosome Content and in Synthesis of Proteins in Leaves of Capsicum annuum L

Barrie T Steer 1,2,1, Graeme C Blackwood 1,2
PMCID: PMC1092252  PMID: 16660636

Abstract

Capsicum annuum cv. California Wonder was grown in controlled environment with a 12-hour photoperiod. The polyribosome content of leaves varied from 60 to 72% of total ribosomes with the highest level occurring in the middle of the photoperiod and the lowest in the middle of the dark period. The variation was accounted for by changes in the content of large polyribosomes (hexamers and larger). There was no indication of an immediate effect on polyribosome content of light-on or light-off.

The synthesis of proteins at two times in the 24-hour cycle was compared using a dual isotope technique. Statistically significant results were obtained that suggested that protein(s) with molecular weights of 26,000 daltons were preferentially synthesized in the photoperiod compared to the dark period. No evidence was found for the differential synthesis of proteins within the photoperiod.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apel K., Kloppstech K. The plastid membranes of barley (Hordeum vulgare). Light-induced appearance of mRNA coding for the apoprotein of the light-harvesting chlorophyll a/b protein. Eur J Biochem. 1978 Apr 17;85(2):581–588. doi: 10.1111/j.1432-1033.1978.tb12273.x. [DOI] [PubMed] [Google Scholar]
  2. Armond P. A., Staehelin L. A., Arntzen C. J. Spatial relationship of photosystem I, photosystem II, and the light-harvesting complex in chloroplast membranes. J Cell Biol. 1977 May;73(2):400–418. doi: 10.1083/jcb.73.2.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLARK M. F., MATTHEWS R. E., RALPH R. K. RIBOSOMES AND POLYRIBOSOMES IN BRASSICA PEKINENSIS. Biochim Biophys Acta. 1964 Oct 16;91:289–304. doi: 10.1016/0926-6550(64)90253-1. [DOI] [PubMed] [Google Scholar]
  4. Davies E., Larkins B. A., Knight R. H. Polyribosomes from peas: an improved method for their isolation in the absence of ribonuclease inhibitors. Plant Physiol. 1972 Nov;50(5):581–584. doi: 10.1104/pp.50.5.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davies E., Larkins B. A. Polyribosomes from Peas: II. Polyribosome Metabolism during Normal and Hormone-induced Growth. Plant Physiol. 1973 Oct;52(4):339–345. doi: 10.1104/pp.52.4.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jackson A. O., Larkins B. A. Influence of Ionic Strength, pH, and Chelation of Divalent Metals on Isolation of Polyribosomes from Tobacco Leaves. Plant Physiol. 1976 Jan;57(1):5–10. doi: 10.1104/pp.57.1.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jacquez J. A., Foster D. M., Shanahan M. F. Analysis of data from experiments using double labeling. Anal Biochem. 1978 Jan;84(1):19–36. doi: 10.1016/0003-2697(78)90480-3. [DOI] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Leaver C. J., Dyer J. A. Caution in the interpretation of plant ribosome studies. Biochem J. 1974 Oct;144(1):165–167. doi: 10.1042/bj1440165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Smith H. Phytochrome-mediated assembly of polyribosomes in etiolated bean leaves. Evidence for post-transciptional regulation of development. Eur J Biochem. 1976 May 17;65(1):161–170. doi: 10.1111/j.1432-1033.1976.tb10401.x. [DOI] [PubMed] [Google Scholar]
  11. Steer B. T. Control of Diurnal Variations in Photosynthetic Products: II. Nitrate Reductase Activity. Plant Physiol. 1974 Nov;54(5):762–765. doi: 10.1104/pp.54.5.762. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Steer B. T. Control of diurnal variations in photosynthetic products: I. Carbon metabolism. Plant Physiol. 1974 Nov;54(5):758–761. doi: 10.1104/pp.54.5.758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Steer B. T. Diurnal variations in photosynthetic products and nitrogen metabolism in expanding leaves. Plant Physiol. 1973 Apr;51(4):744–748. doi: 10.1104/pp.51.4.744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Steer B. T. Rhythmic Nitrate Reductase Activity in Leaves of Capsicum annuum L. and the Influence of Kinetin. Plant Physiol. 1976 Jun;57(6):928–932. doi: 10.1104/pp.57.6.928. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Travis R. L., Huffaker R. C. Light-induced Development of Polyribosomes and the Induction of Nitrate Reductase in Corn Leaves. Plant Physiol. 1970 Dec;46(6):800–805. doi: 10.1104/pp.46.6.800. [DOI] [PMC free article] [PubMed] [Google Scholar]

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