Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1978 Dec;62(6):949–953. doi: 10.1104/pp.62.6.949

Effect of Plant Growth Regulators on Calcium-stimulated Serine Transport into Tobacco Cells

Ivan K Smith 1
PMCID: PMC1092262  PMID: 16660646

Abstract

The transport of serine into tobacco cells (Nicotiana tabacum L.) cultured in liquid medium was examined. Transport was inhibited approximately 50% by 2,4-dichlorophenoxyacetic acid, indoleacetic acid, α-naphthalene acetic acid, and kinetin at a concentration of 10 micrograms per milliliter. Transport was not inhibited by 2,6-dichlorophenoxyacetic acid and inhibited less than 25% by p-chlorophenoxyacetic acid at this concentration. Removal of 2,4-dichlorophenoxyacetic acid from the transport medium resulted in an alleviation of inhibition. Gibberellic acid at concentrations from 2 to 20 micrograms per milliliter stimulated transport.

It was previously shown that inhibition of transport by La3+ was due to removal of Ca2+ from surface sites and inhibition of Ca2+ uptake by cells. None of the growth regulators tested had any significant effect on Ca2+ binding and/or transport.

A contributing factor to the low transport rates in the absence of Ca2+ is the increased rate of serine efflux. None of the growth regulators tested had any significant effect on the rate of serine efflux.

Full text

PDF
949

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cleland R. E. Reevaluation of the Effect of Calcium Ions on Auxin-induced Elongation. Plant Physiol. 1977 Nov;60(5):709–712. doi: 10.1104/pp.60.5.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gamborg O. L. The effects of amino acids and ammonium on the growth of plant cells in suspension culture. Plant Physiol. 1970 Apr;45(4):372–375. doi: 10.1104/pp.45.4.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Harrington H. M., Smith I. K. Cysteine transport into cultured tobacco cells. Plant Physiol. 1977 Dec;60(6):807–811. doi: 10.1104/pp.60.6.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Helgerson S. L., Cramer W. A., Morré D. J. Evidence for an increase in microviscosity of plasma membranes from soybean hypocotyls induced by the plant hormone, indole-3-acetic Acid. Plant Physiol. 1976 Oct;58(4):548–551. doi: 10.1104/pp.58.4.548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LeJohn H. B., Cameron L. E. Cytokinins regulate calcium binding to a glycoprotein from fungal cells. Biochem Biophys Res Commun. 1973 Oct 1;54(3):1053–1060. doi: 10.1016/0006-291x(73)90800-0. [DOI] [PubMed] [Google Scholar]
  6. LeJohn H. B., Stevenson R. M. Cytokinins and magnesium ions may control the flow of metabolites and calcium ions through fungal cell membranes. Biochem Biophys Res Commun. 1973 Oct 1;54(3):1061–1066. doi: 10.1016/0006-291x(73)90801-2. [DOI] [PubMed] [Google Scholar]
  7. Morré D. J., Bracker C. E. Ultrastructural alteration of plant plasma membranes induced by auxin and calcium ions. Plant Physiol. 1976 Oct;58(4):544–547. doi: 10.1104/pp.58.4.544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rubinstein B. Osmotic Shock Inhibits Auxin-stimulated Acidification and Growth. Plant Physiol. 1977 Mar;59(3):369–371. doi: 10.1104/pp.59.3.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Singh D. P., LéJohn H. B. Amino acid transport in a water-mould: the possible regulatory roles of calcium and N6-(delta2-isopentenyl)adenine. Can J Biochem. 1975 Sep;53(9):975–988. doi: 10.1139/o75-134. [DOI] [PubMed] [Google Scholar]
  10. Smith I. K. Role of calcium in serine transport into tobacco cells. Plant Physiol. 1978 Dec;62(6):941–948. doi: 10.1104/pp.62.6.941. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES