Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1978 Dec;62(6):978–980. doi: 10.1104/pp.62.6.978

Phosphatidylinositol Synthesis in Castor Bean Endosperm

Cytidine Diphosphate Diglyceride:Inositol Transferase 1

James C Sexton 1, Thomas S Moore Jr 1
PMCID: PMC1092266  PMID: 16660650

Abstract

CDP-diglyceride:inositol transferase in endoplasmic reticulum fractions from castor bean (Ricinus communis) endosperm was partially characterized. The enzyme had a pH optimum of 8.5 and required Mn2+ for activity. Maximal activity was at 1.5 millimolar MnCl2. A Km of 0.30 mM was calculated for myo-inositol and 1.35 millimolar was estimated for CDP-dipalmitoylglyceride. Concentrations of CDP-dipalmitoylglyceride above 1.2 millimolar inhibited the enzyme. A deoxycholate concentration of 0.1% (w/v) stimulated the reaction slightly while Triton X-100 inhibited at all concentrations tested. Some incorporation of myo-inositol into phosphatidylinositol occurred in the absence of CDP-diglyceride.

Full text

PDF
978

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agranoff B. W., Benjamin J. A., Hajra A. K. Biosynthesis of phosphatidylinositol. Ann N Y Acad Sci. 1969 Oct 17;165(2):755–760. doi: 10.1111/j.1749-6632.1970.tb55954.x. [DOI] [PubMed] [Google Scholar]
  2. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  3. Benjamins J. A., Agranoff B. W. Distribution and properties of CDP-diglyceride:inositol transferase from brain. J Neurochem. 1969 Apr;16(4):513–527. doi: 10.1111/j.1471-4159.1969.tb06850.x. [DOI] [PubMed] [Google Scholar]
  4. Holub B. J. The Mn2+-activated incorporation of inositol into molecular species of phosphatidylinositol in rat liver microsomes. Biochim Biophys Acta. 1974 Oct 16;369(1):111–122. doi: 10.1016/0005-2760(74)90197-0. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Moore T. S., Lord J. M., Kagawa T., Beevers H. Enzymes of phospholipid metabolism in the endoplasmic reticulum of castor bean endosperm. Plant Physiol. 1973 Jul;52(1):50–53. doi: 10.1104/pp.52.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moore T. S. Phosphatidylserine synthesis in castor bean endosperm. Plant Physiol. 1975 Aug;56(2):177–180. doi: 10.1104/pp.56.2.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PAULUS H., KENNEDY E. P. The enzymatic synthesis of inositol monophosphatide. J Biol Chem. 1960 May;235:1303–1311. [PubMed] [Google Scholar]
  9. Prottey C., Hawthorne J. N. The biosynthesis of phosphatidic acid and phosphatidylinositol in mammalian pancreas. Biochem J. 1967 Oct;105(1):379–392. doi: 10.1042/bj1050379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sumida S., Mudd J. B. The structure and biosynthesis of phosphatidyl inositol in cauliflower inflorescence. Plant Physiol. 1970 Jun;45(6):712–718. doi: 10.1104/pp.45.6.712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Takenawa T., Egawa K. CDP-diglyceride:inositol transferase from rat liver. Purification and properties. J Biol Chem. 1977 Aug 10;252(15):5419–5423. [PubMed] [Google Scholar]
  12. Takenawa T., Saito M., Nagai Y., Egawa K. Solubilization of the enzyme catalyzing CDP-diglyceride-independent incorporation of myo-inositol into phosphatidyl inositol and its comparison to CDP-diglyceride:inositol transferase. Arch Biochem Biophys. 1977 Jul;182(1):244–250. doi: 10.1016/0003-9861(77)90304-6. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES