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. 1978 Dec;62(6):987–990. doi: 10.1104/pp.62.6.987

Nitrate Utilization by the Diatom Skeletonema costatum

I. Kinetics of Nitrate Uptake 1

Juan L Serra 1,2, Maria J Llama 1,2, Eduardo Cadenas 1,3
PMCID: PMC1092268  PMID: 16660652

Abstract

Nitrate uptake has been studied in nitrogen-deficient cells of the marine diatom Skeletonema costatum. When these cells are incubated in the presence of nitrate, this ion is quickly taken up from the medium, and nitrite is excreted by the cells. Nitrite is excreted following classical saturation kinetics, its rate being independent of nitrate concentration in the incubation medium for nitrate concentration values higher than 3 micromolar. Nitrate uptake shows mixed-transfer kinetics, which can be attributed to the simultaneous contributions of mediated and diffusion transfer. Cycloheximide and p-hydroxymercuribenzoate inhibit the carrier-mediated contribution to nitrate uptake, without affecting the diffusion component. When cells are preincubated with nitrate, the net nitrogen uptake is increased.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. GUILLARD R. R., RYTHER J. H. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (cleve) Gran. Can J Microbiol. 1962 Apr;8:229–239. doi: 10.1139/m62-029. [DOI] [PubMed] [Google Scholar]
  2. Goldsmith J., Livoni J. P., Norberg C. L., Segel I. H. Regulation of Nitrate Uptake in Penicillium chrysogenum by Ammonium Ion. Plant Physiol. 1973 Oct;52(4):362–367. doi: 10.1104/pp.52.4.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Jackson W. A., Flesher D., Hageman R. H. Nitrate Uptake by Dark-grown Corn Seedlings: Some Characteristics of Apparent Induction. Plant Physiol. 1973 Jan;51(1):120–127. doi: 10.1104/pp.51.1.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Neyra C. A., Hageman R. H. Nitrate uptake and induction of nitrate reductase in excised corn roots. Plant Physiol. 1975 Nov;56(5):692–695. doi: 10.1104/pp.56.5.692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Rao K. P., Rains D. W. Nitrate Absorption by Barley: II. Influence of Nitrate Reductase Activity. Plant Physiol. 1976 Jan;57(1):59–62. doi: 10.1104/pp.57.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Rao K. P., Rains D. W. Nitrate absorption by barley: I. Kinetics and energetics. Plant Physiol. 1976 Jan;57(1):55–58. doi: 10.1104/pp.57.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ryther J. H., Dunstan W. M. Nitrogen, phosphorus, and eutrophication in the coastal marine environment. Science. 1971 Mar 12;171(3975):1008–1013. doi: 10.1126/science.171.3975.1008. [DOI] [PubMed] [Google Scholar]

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